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Cultured Human Epidermis Combined With Meshed Skin Autografts Accelerates Epithelialization and Granulation Tissue Formation in a Rat Model
- Source :
- Annals of Plastic Surgery
- Publication Year :
- 2017
- Publisher :
- Lippincott Williams and Wilkins, 2017.
-
Abstract
- INTRODUCTION: As the take rate of cultured epidermal autografts in burn wound treatment is variable, widely expanded meshed auto skin grafts are often used in combination with cultured epidermal autograft to increase the take rate and achieve definitive wound coverage. However, a long time (3–4 weeks) required to prepare a cultured epidermis sheet is a disadvantage. Allogeneic cultured epidermis can be prepared in advance and cryopreserved to be used in combination with auto meshed skin grafts for treating third-degree burns. Nevertheless, the human cultured epidermis (hCE) has not been proved to accelerate wound healing after meshed skin grafting. Here, we investigated the effect of hCE on wound healing in a rat model of meshed skin grafting. MATERIALS AND METHODS: Human cultured epidermis was prepared from human neonatal foreskin and assessed by the release of growth factors into the culture medium using enzyme-linked immunosorbent assay. Skin wounds were inflicted on male F344 rats and treated by the application of widely meshed (6:1 ratio) autogenous skin grafts with or without hCE (n = 8 rats per group). Wound area, neoepithelium length, granulation tissue formation, and neovascularization were evaluated on day 7 postgrafting. RESULTS: Human cultured epidermis secreted IL-1α, Basic fibroblast growth factor, platelet-derived growth factor-AA, TGF-α, TGF-β1, and vascular endothelial growth factor in vitro. In rats, hCE accelerated wound closure (P = 0.003), neoepithelium growth (P = 0.019), and granulation tissue formation (P = 0.043), and increased the number of capillaries (P = 0.0003) and gross neovascularization area (P = 0.008) compared with the control group. CONCLUSIONS: The application of hCE with meshed grafts promoted wound closure, possibly via secretion of growth factors critical for cell proliferation and migration, suggesting that hCE can enhance the healing effect of widely expanded skin autografts.This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.
- Subjects :
- Male
Burn Surgery and Research
Pathology
medicine.medical_specialty
medicine.medical_treatment
Basic fibroblast growth factor
Enzyme-Linked Immunosorbent Assay
wound healing
cultured epidermis
Neovascularization
030207 dermatology & venereal diseases
03 medical and health sciences
chemistry.chemical_compound
Foreskin
0302 clinical medicine
Re-Epithelialization
medicine
Animals
Humans
Autografts
Epidermis (botany)
integumentary system
business.industry
Growth factor
rat model
Granulation tissue
030208 emergency & critical care medicine
growth factor
Skin Transplantation
Rats, Inbred F344
Surgery
Rats
meshed skin graft
Disease Models, Animal
medicine.anatomical_structure
chemistry
Epidermal Cells
Granulation Tissue
Skin grafting
ELISA
sense organs
medicine.symptom
business
Wound healing
Burns
Subjects
Details
- Language :
- English
- ISSN :
- 15363708
- Volume :
- 78
- Database :
- OpenAIRE
- Journal :
- Annals of Plastic Surgery
- Accession number :
- edsair.doi.dedup.....d4b20310fdb93f4dddecfafa1102ae91