Back to Search
Start Over
PGE2-mediated podosome loss in dendritic cells is dependent on actomyosin contraction downstream of the RhoA-Rho-kinase axis
- Source :
- Journal of Cell Science, 121, 1096-106, Journal of Cell Science, 121, Pt 7, pp. 1096-106
- Publication Year :
- 2008
-
Abstract
- Item does not contain fulltext Podosomes are dynamic adhesion structures found in dendritic cells (DCs) and other cells of the myeloid lineage. We previously showed that prostaglandin E2 (PGE2), an important proinflammatory mediator produced during DC maturation, induces podosome disassembly within minutes after stimulation. Here, we demonstrate that this response is mediated by cAMP elevation, occurs downstream of Rho kinase and is dependent on myosin II. Whereas PGE2 stimulation leads to activation of the small GTPase RhoA, decreased levels of Rac1-GTP and Cdc42-GTP are observed. These results show that PGE2 stimulation leads to activation of the RhoA-Rho-kinase axis to promote actomyosin-based contraction and subsequent podosome dissolution. Because podosome disassembly is accompanied by de novo formation of focal adhesions, we propose that the disassembly/formation of these two different adhesion structures is oppositely regulated by actomyosin contractility and relative activities of RhoA, Rac1 and Cdc42.
- Subjects :
- rac1 GTP-Binding Protein
RHOA
Age-related aspects of cancer [ONCOL 2]
Podosome
RAC1
HL-60 Cells
CDC42
Biology
Models, Biological
Dinoprostone
Focal adhesion
Immune Regulation [NCMLS 2]
Translational research [ONCOL 3]
Myosin
Cyclic AMP
Humans
Receptors, Prostaglandin E
Small GTPase
cdc42 GTP-Binding Protein
Rho-associated protein kinase
Cells, Cultured
Reverse Transcriptase Polymerase Chain Reaction
Immunotherapy, gene therapy and transplantation [UMCN 1.4]
Cell Biology
Actomyosin
Dendritic Cells
Cell biology
Microscopy, Fluorescence
biology.protein
rhoA GTP-Binding Protein
Signal Transduction
Immunity, infection and tissue repair [NCMLS 1]
Subjects
Details
- ISSN :
- 00219533
- Volume :
- 121
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Science
- Accession number :
- edsair.doi.dedup.....d4b178cbe678b0ca49b030bc2575efbf