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Coordinate Induction of Humoral and Spike Specific T-Cell Response in a Cohort of Italian Health Care Workers Receiving BNT162b2 mRNA Vaccine

Authors :
Chiara Agrati
Concetta Castilletti
Delia Goletti
Silvia Meschi
Alessandra Sacchi
Giulia Matusali
Veronica Bordoni
Linda Petrone
Daniele Lapa
Stefania Notari
Valentina Vanini
Francesca Colavita
Alessandra Aiello
Alessandro Agresta
Chiara Farroni
Germana Grassi
Sara Leone
Francesco Vaia
Maria Rosaria Capobianchi
Giuseppe Ippolito
Vincenzo Puro
on behalf of the INMI COVID-190 Vaccine Study Group
Agrati, C.
Castilletti, C.
Goletti, D.
Meschi, S.
Sacchi, A.
Matusali, G.
Bordoni, V.
Petrone, L.
Lapa, D.
Notari, S.
Vanini, V.
Colavita, F.
Aiello, A.
Agresta, A.
Farroni, C.
Grassi, G.
Leone, S.
Vaia, F.
Capobianchi, M. R.
Ippolito, G.
Puro, V.
Source :
Microorganisms, Microorganisms, Vol 9, Iss 1315, p 1315 (2021)
Publication Year :
2021
Publisher :
MDPI, 2021.

Abstract

Vaccination is the main public health measure to reduce SARS-CoV-2 transmission and hospitalization, and a massive worldwide scientific effort resulted in the rapid development of effective vaccines. This work aimed to define the dynamics of humoral and cell-mediated immune response in a cohort of health care workers (HCWs) who received a two-dose BNT162b2-mRNA vaccination. The serological response was evaluated by quantifying the anti-RBD and neutralizing antibodies. The cell-mediated response was performed by a whole blood test quantifying Th1 cytokines (IFN-γ, TNF-α, IL-2), produced in response to spike peptides. The BNT162b2-mRNA vaccine induced both humoral and cell-mediated immune responses against spike peptides in virtually all HCWs without previous SARS-CoV-2 infection, with a moderate inverse relation with age in the anti-RBD response. Spike-specific T cells produced several Th1 cytokines (IFN-γ, TNF-α, and IL-2), which correlated with the specific-serological response. Overall, our study describes the ability of the BNT162b2 mRNA vaccine to elicit a coordinated neutralizing humoral and spike-specific T cell response in HCWs. Assessing the dynamics of these parameters by an easy immune monitoring protocol can allow for the evaluation of the persistence of the vaccine response in order to define the optimal vaccination strategy.

Details

Language :
English
ISSN :
20762607
Volume :
9
Issue :
6
Database :
OpenAIRE
Journal :
Microorganisms
Accession number :
edsair.doi.dedup.....d487461412297a3abe678ef3311423d6