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Potential for alternative intron–exon pairings in group II intron RmInt1 from Sinorhizobium meliloti and its relatives
- Source :
- RNA, RNA, Cold Spring Harbor Laboratory Press, 2006, 12 (3), pp.338-41. ⟨10.1261/rna.2240906⟩
- Publication Year :
- 2006
- Publisher :
- Cold Spring Harbor Laboratory, 2006.
-
Abstract
- Ribozyme constructs derived from group II intron RmInt1 of Sinorhizobium meliloti self-splice in vitro when incubated under permissive conditions, but exon ligation is unusually inefficient when the 5′ exon is truncated close to the IBS2 intron-binding site. One plausible explanation for this observation is the presence of an alternative intron–exon pairing between an intron segment that overlaps with the EBS2 exon-binding site and a 5′ exon site located just distal of IBS2 relative to the splice junction. Strikingly, the existence of this pairing is supported by comparative sequence analysis of introns related to RmInt1.
- Subjects :
- Models, Molecular
MESH: Introns
Sequence analysis
Molecular Sequence Data
MESH: Base Sequence
03 medical and health sciences
Exon
MESH: RNA, Catalytic
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
RNA, Catalytic
Letter to the Editor
Molecular Biology
030304 developmental biology
Genetics
0303 health sciences
Sinorhizobium meliloti
MESH: Molecular Sequence Data
Binding Sites
Splice site mutation
Base Sequence
biology
030306 microbiology
MESH: Alternative Splicing
Alternative splicing
Ribozyme
Intron
Exons
Group II intron
biology.organism_classification
Molecular biology
Introns
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Alternative Splicing
RNA, Bacterial
MESH: Nucleic Acid Conformation
MESH: Binding Sites
biology.protein
Nucleic Acid Conformation
MESH: Exons
MESH: RNA, Bacterial
MESH: Sinorhizobium meliloti
MESH: Models, Molecular
Subjects
Details
- ISSN :
- 14699001 and 13558382
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- RNA
- Accession number :
- edsair.doi.dedup.....d470b32615756ee0c227c665b07e5ca2
- Full Text :
- https://doi.org/10.1261/rna.2240906