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Potential for alternative intron–exon pairings in group II intron RmInt1 from Sinorhizobium meliloti and its relatives

Authors :
Nicolás Toro
François Michel
Maria do Carmo Costa
Centre de génétique moléculaire (CGM)
Centre National de la Recherche Scientifique (CNRS)
Grupo de Ecologıa Genética, Estacion, Granada
Consejo Superior de Investigaciones Científicas [Madrid] (CSIC)
Source :
RNA, RNA, Cold Spring Harbor Laboratory Press, 2006, 12 (3), pp.338-41. ⟨10.1261/rna.2240906⟩
Publication Year :
2006
Publisher :
Cold Spring Harbor Laboratory, 2006.

Abstract

Ribozyme constructs derived from group II intron RmInt1 of Sinorhizobium meliloti self-splice in vitro when incubated under permissive conditions, but exon ligation is unusually inefficient when the 5′ exon is truncated close to the IBS2 intron-binding site. One plausible explanation for this observation is the presence of an alternative intron–exon pairing between an intron segment that overlaps with the EBS2 exon-binding site and a 5′ exon site located just distal of IBS2 relative to the splice junction. Strikingly, the existence of this pairing is supported by comparative sequence analysis of introns related to RmInt1.

Details

ISSN :
14699001 and 13558382
Volume :
12
Database :
OpenAIRE
Journal :
RNA
Accession number :
edsair.doi.dedup.....d470b32615756ee0c227c665b07e5ca2
Full Text :
https://doi.org/10.1261/rna.2240906