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Comparative proteomics reveals human pluripotent stem cell-derived limbal epithelial stem cells are similar to native ocular surface epithelial cells
- Source :
- Scientific Reports
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Limbal epithelial stem cells (LESCs) are tissue-specific stem cells responsible for renewing the corneal epithelium. Acute trauma or chronic disease affecting LESCs may disrupt corneal epithelial renewal, causing vision threatening and painful ocular surface disorders, collectively referred to as LESC deficiency (LESCD). These disorders cannot be treated with traditional corneal transplantation and therefore alternative cell sources for successful cell-based therapy are needed. LESCs derived from human pluripotent stem cells (hPSCs) are a prospective source for ocular surface reconstruction, yet critical evaluation of these cells is crucial before considering clinical applications. In order to quantitatively evaluate hPSC-derived LESCs, we compared protein expression in native human corneal cells to that in hPSC-derived LESCs using isobaric tag for relative and absolute quantitation (iTRAQ) technology. We identified 860 unique proteins present in all samples, including proteins involved in cell cycling, proliferation, differentiation and apoptosis, various LESC niche components and limbal and corneal epithelial markers. Protein expression profiles were nearly identical in LESCs derived from two different hPSC lines, indicating that the differentiation protocol is reproducible, yielding homogeneous cell populations. Their protein expression profile suggests that hPSC-derived LESCs are similar to the human ocular surface epithelial cells and possess LESC-like characteristics.
- Subjects :
- Male
Pluripotent Stem Cells
Proteomics
Pathology
medicine.medical_specialty
Proteome
Cellular differentiation
Human Embryonic Stem Cells
Cell
Biology
Article
Cell Line
medicine
Humans
Stem Cell Niche
Induced pluripotent stem cell
Aged
Cell Proliferation
Corneal epithelium
Multidisciplinary
Biolääketieteet – Biomedicine
Cell growth
Epithelium, Corneal
Cell Differentiation
Epithelial Cells
Korva-, nenä- ja kurkkutaudit, silmätaudit - Otorhinolaryngology, ophthalmology
Embryonic stem cell
eye diseases
3. Good health
Cell biology
medicine.anatomical_structure
Cell culture
sense organs
Stem cell
Apoptosis Regulatory Proteins
Biomarkers
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....d4542cc2daefaf28b15b295daa9889d6