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β-aminoisobutyric acid attenuates LPS-induced inflammation and insulin resistance in adipocytes through AMPK-mediated pathway
- Source :
- Journal of Biomedical Science, Journal of Biomedical Science, Vol 25, Iss 1, Pp 1-9 (2018)
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Background β-aminoisobutyric acid (BAIBA) is produced in skeletal muscle during exercise and has beneficial effects on obesity-related metabolic disorders such as diabetes and non-alcoholic fatty liver disease. Thus, it is supposed to prevent high fat diet (HFD)-induced inflammation and insulin resistance in adipose tissue though anti-inflammatory effects in obesity. Previous reports have also demonstrated strong anti-inflammatory effects of BAIBA. Methods We used BAIBA treated fully differentiated 3T3T-L1 mouse adipocytes to investigate the effects of exogenous BAIBA on inflammation and insulin signaling in adipocytes. Insulin signaling-mediated proteins and inflammation markers were measured by Western blot analysis. Secretion of pro-inflammatory cytokines were measured by ELISA. Lipid accumulation in differentiated 3 T3-L1 cells was stained by Oil red-O. Statistical analysis was performed by ANOVA and student’s t test. Results BAIBA treatment suppressed adipogenesis assessed by adipogenic markers as well as lipid accumulation after full differentiation. We showed that BAIBA treatment stimulated AMP-activated protein kinase (AMPK) phosphorylation in a dose-dependent manner and lipopolysaccharide (LPS)-induced secretion of pro-inflammatory cytokines such as TNFα and MCP-1 was abrogated in BAIBA-treated 3 T3-L1 cells. Treatment of 3 T3-L1 cells with BAIBA reduced LPS-induced NFκB and IκB phosphorylation. Furthermore, BAIBA treatment ameliorated LPS-induced impairment of insulin signaling measured by IRS-1 and Akt phosphorylation and fatty acid oxidation. Suppression of AMPK by small interfering (si) RNA significantly restored these changes. Conclusions We demonstrated anti-inflammatory and anti-insulin resistance effects of BAIBA in differentiated 3 T3-L1 cells treated with LPS through AMPK-dependent signaling. These results provide evidence for the beneficial effects of BAIBA not only in liver and skeletal muscle cells but also in adipose tissue. Electronic supplementary material The online version of this article (10.1186/s12929-018-0431-7) contains supplementary material, which is available to authorized users.
- Subjects :
- AMPK
Lipopolysaccharides
0301 basic medicine
medicine.medical_specialty
Aminoisobutyric Acids
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Clinical Biochemistry
lcsh:Medicine
Adipose tissue
AMP-Activated Protein Kinases
Mice
03 medical and health sciences
chemistry.chemical_compound
Insulin resistance
AMP-activated protein kinase
3T3-L1 Cells
Adipocyte
Internal medicine
Adipocytes
medicine
Animals
Pharmacology (medical)
Molecular Biology
Inflammation
biology
Research
Insulin
lcsh:R
Biochemistry (medical)
Cell Biology
General Medicine
medicine.disease
BAIBA
Insulin receptor
030104 developmental biology
Endocrinology
chemistry
Adipogenesis
biology.protein
NFκB
Signal Transduction
Subjects
Details
- ISSN :
- 14230127
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Science
- Accession number :
- edsair.doi.dedup.....d4472935862f67dcc44cff8c8bae45bc