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Bovine Doppel (Dpl) and prion protein (PrP) expression on lymphoid tissue and circulating leukocytes
- Source :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 52(12)
- Publication Year :
- 2004
-
Abstract
- Doppel (Dpl) protein shares some structural features with prion protein (PrP), whose pathologic isoform (PrPsc) is considered to be the causative agent of transmissible spongiform encephalopathies. Dpl is mainly expressed in testes but, when ectopically expressed in the central nervous system, is neurotoxic. We have examined the expression pattern of Dpl and PrP on bovine lymphoid tissues and circulating leukocytes. A polyclonal anti-Dpl antibody along with a panel of monoclonal antibodies specific for leukocyte membrane antigens or PrP were used to examine frozen sections from spleen, lymph nodes, and bone marrow by immunohistochemistry. Blood was analyzed by flow cytometry. Double staining was used to study the possible coexpression of the two proteins and to characterize cells expressing Dpl and/or PrP. Dpl was expressed in B-cells, in dendritic cells within lymphoid follicles, bone marrow, circulating myeloid cells, and circulating B-cells. The distribution of Dpl was quite similar to that of PrP. The only differences in expression observed concerned the low number of Dpl + cells in lymph nodes and the strong Dpl positivity of circulating granulocytes. The two proteins were rarely co-expressed, suggesting an independent expression mechanism in resting cells. The role of Dpl+ leukocytes in the pathogenesis of Dpl- or PrP-induced diseases merits further investigation.
- Subjects :
- 0301 basic medicine
Male
Pathology
medicine.medical_specialty
Histology
medicine.drug_class
Lymphoid Tissue
Prions
Spleen
Monoclonal antibody
03 medical and health sciences
Antigen
Bone Marrow
medicine
Leukocytes
Animals
030102 biochemistry & molecular biology
biology
Follicular dendritic cells
Flow Cytometry
Molecular biology
Immunohistochemistry
030104 developmental biology
medicine.anatomical_structure
Lymphatic system
Blood Circulation
biology.protein
Cattle
Female
Bone marrow
Lymph Nodes
Anatomy
Antibody
Subjects
Details
- ISSN :
- 00221554
- Volume :
- 52
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
- Accession number :
- edsair.doi.dedup.....d4447fff524af2124d13e63b00153276