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Growth of Epithelial Organoids in a Defined Hydrogel
- Source :
- Advanced Materials, 30 (43)
- Publication Year :
- 2018
- Publisher :
- Wiley-VCH, 2018.
-
Abstract
- Epithelial organoids are simplified models of organs grown in vitro from embryonic and adult stem cells. They are widely used to study organ development and disease, and enable drug screening in patient‐derived primary tissues. Current protocols, however, rely on animal‐ and tumor‐derived basement membrane extract (BME) as a 3D scaffold, which limits possible applications in regenerative medicine. This prompted us to study how organoids interact with their matrix, and to develop a well‐defined hydrogel that supports organoid generation and growth. It is found that soft fibrin matrices provide suitable physical support, and that naturally occurring Arg‐Gly‐Asp (RGD) adhesion domains on the scaffold, as well as supplementation with laminin‐111, are key parameters required for robust organoid formation and expansion. The possibility to functionalize fibrin via factor XIII‐mediated anchoring also allows to covalently link fluorescent nanoparticles to the matrix for 3D traction force microscopy. These measurements suggest that the morphogenesis of budding intestinal organoids results from internal pressure combined with higher cell contractility in the regions containing differentiated cells compared to the regions containing stem cells. Since the fibrin/laminin matrix supports long‐term expansion of all tested murine and human epithelial organoids, this hydrogel can be widely used as a defined equivalent to BME. ISSN:0935-9648 ISSN:1521-4095
- Subjects :
- 0301 basic medicine
Materials science
Surface Properties
Cellular differentiation
2210 Mechanical Engineering
Mice, Transgenic
610 Medicine & health
02 engineering and technology
Traction force microscopy
Regenerative medicine
Epithelium
Cell Line
Tissue Culture Techniques
03 medical and health sciences
2211 Mechanics of Materials
Intestine, Small
Cell Adhesion
Organoid
Animals
Humans
General Materials Science
Pancreas
Fibrin
Tissue Scaffolds
Stem Cells
Mechanical Engineering
Hydrogels
021001 nanoscience & nanotechnology
Embryonic stem cell
2500 General Materials Science
3. Good health
Cell biology
Mice, Inbred C57BL
Organoids
10219 Clinic for Gastroenterology and Hepatology
030104 developmental biology
Liver
Mechanics of Materials
fibrin
hydrogels
organoids
Self-healing hydrogels
Laminin
Stem cell
0210 nano-technology
Adult stem cell
Subjects
Details
- Language :
- English
- ISSN :
- 09359648
- Database :
- OpenAIRE
- Journal :
- Advanced Materials, 30 (43)
- Accession number :
- edsair.doi.dedup.....d444014b0429ebfd6757cf7218238100