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Quantitative Proteomics Identifies Serum Response Factor Binding Protein 1 as a Host Factor for Hepatitis C Virus Entry
- Source :
- Cell Reports, Vol 12, Iss 5, Pp 864-878 (2015), Cell Reports, CELL REPORTS
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- SummaryHepatitis C virus (HCV) enters human hepatocytes through a multistep mechanism involving, among other host proteins, the virus receptor CD81. How CD81 governs HCV entry is poorly characterized, and CD81 protein interactions after virus binding remain elusive. We have developed a quantitative proteomics protocol to identify HCV-triggered CD81 interactions and found 26 dynamic binding partners. At least six of these proteins promote HCV infection, as indicated by RNAi. We further characterized serum response factor binding protein 1 (SRFBP1), which is recruited to CD81 during HCV uptake and supports HCV infection in hepatoma cells and primary human hepatocytes. SRFBP1 facilitates host cell penetration by all seven HCV genotypes, but not of vesicular stomatitis virus and human coronavirus. Thus, SRFBP1 is an HCV-specific, pan-genotypic host entry factor. These results demonstrate the use of quantitative proteomics to elucidate pathogen entry and underscore the importance of host protein-protein interactions during HCV invasion.
- Subjects :
- Proteomics
Receptor complex
viruses
Hepatitis C virus
Quantitative proteomics
Hepacivirus
Sciences du Vivant [q-bio]/MĂ©decine humaine et pathologie
Biology
medicine.disease_cause
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Cell Line, Tumor
medicine
Humans
lcsh:QH301-705.5
030304 developmental biology
Host factor
0303 health sciences
Virus receptor
030302 biochemistry & molecular biology
virus diseases
Virus Internalization
biology.organism_classification
Virology
digestive system diseases
3. Good health
NS2-3 protease
lcsh:Biology (General)
Vesicular stomatitis virus
Transcription Factors
CD81
Subjects
Details
- ISSN :
- 22111247
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Cell Reports
- Accession number :
- edsair.doi.dedup.....d441864896134d2e3f020bea48d8cd85