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SadA, a Trimeric Autotransporter from Salmonella enterica Serovar Typhimurium, Can Promote Biofilm Formation and Provides Limited Protection against Infection
- Source :
- Infection and Immunity. 79:4342-4352
- Publication Year :
- 2011
- Publisher :
- American Society for Microbiology, 2011.
-
Abstract
- Salmonella enterica is a major cause of morbidity worldwide and mortality in children and immunocompromised individuals in sub-Saharan Africa. Outer membrane proteins of Salmonella are of significance because they are at the interface between the pathogen and the host, they can contribute to adherence, colonization, and virulence, and they are frequently targets of antibody-mediated immunity. In this study, the properties of SadA, a purported trimeric autotransporter adhesin of Salmonella enterica serovar Typhimurium, were examined. We demonstrated that SadA is exposed on the Salmonella cell surface in vitro and in vivo during infection of mice. Expression of SadA resulted in cell aggregation, biofilm formation, and increased adhesion to human intestinal Caco-2 epithelial cells. Immunization of mice with folded, full-length, purified SadA elicited an IgG response which provided limited protection against bacterial challenge. When anti-SadA IgG titers were enhanced by administering alum-precipitated protein, a modest additional protection was afforded. Therefore, despite SadA having pleiotropic functions, it is not a dominant, protective antigen for antibody-mediated protection against Salmonella .
- Subjects :
- Salmonella typhimurium
Salmonella
Immunology
Virulence
Biology
medicine.disease_cause
Microbiology
Bacterial Adhesion
Mice
medicine
Animals
Humans
Trimeric autotransporter adhesin
Adhesins, Bacterial
Pathogen
Phylogeny
Escherichia coli K12
Biofilm
Membrane Proteins
Gene Expression Regulation, Bacterial
biology.organism_classification
Molecular Pathogenesis
Virology
Cell aggregation
Infectious Diseases
Salmonella enterica
Biofilms
Immunoglobulin G
Alum Compounds
Parasitology
Caco-2 Cells
Bacterial outer membrane
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 79
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....d43872cef5673c8b4c7954a146b0bdcd