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Optimal Solution Volume for Luminal Preservation: A Preclinical Study in Porcine Intestinal Preservation
- Source :
- Transplantation Proceedings. 48:532-535
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Background Rodent studies suggest that luminal solutions alleviate the mucosal injury and prolong intestinal preservation but concerns exist that excessive volumes of luminal fluid may promote tissue edema. Differences in size, structure, and metabolism between rats and humans require studies in large animals before clinical use. Methods Intestinal procurement was performed in 7 pigs. After perfusion with histidine-tryptophan-ketoglutarate (HTK), 40-cm-long segments were cut and filled with 13.5% polyethylene glycol (PEG) 3350 solution as follows: V0 (controls, none), V1 (0.5 mL/cm), V2 (1 mL/cm), V3 (1.5 mL/cm), and V4 (2 mL/cm). Tissue and luminal solutions were sampled after 8, 14, and 24 hours of cold storage (CS). Preservation injury (Chiu score), the apical membrane (ZO-1, brush-border maltase activity), and the electrolyte content in the luminal solution were studied. Results In control intestines, 8-hour CS in HTK solution resulted in minimal mucosal changes (grade 1) that progressed to significant subepithelial edema (grade 3) by 24 hours. During this time, a gradual loss in ZO-1 was recorded, whereas maltase activity remained unaltered. Moreover, variable degrees of submucosal edema were observed. Luminal introduction of high volumes (2 mL/mL) of PEG solution accelerated the development of the subepithelial edema and submucosal edema, leading to worse histology. However, ZO-1 was preserved better over time than in control intestines (no luminal solution). Maltase activity was reduced in intestines receiving luminal preservation. Luminal sodium content decreased in time and did not differ between groups. Conclusions This PEG solution protects the apical membrane and the tight-junction proteins but may favor water absorption and tissue (submucosal) edema, and luminal volumes >2 mL/cm may result in worse intestinal morphology.
- Subjects :
- Male
medicine.medical_specialty
Pathology
Swine
Organ Preservation Solutions
Cold storage
030230 surgery
Biology
Gastroenterology
Potassium Chloride
03 medical and health sciences
Procaine
0302 clinical medicine
Edema
Internal medicine
PEG ratio
medicine
Animals
Mannitol
Cryopreservation
Transplantation
Histology
Organ Preservation
Apical membrane
Intestines
Glucose
030211 gastroenterology & hepatology
Surgery
medicine.symptom
Perfusion
medicine.drug
Subjects
Details
- ISSN :
- 00411345
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Transplantation Proceedings
- Accession number :
- edsair.doi.dedup.....d41731e29523a0cc2138f1d1daaf1cf1