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Protective efficacy of a recombinant bacterial artificial chromosome clone of a very virulent Marek’s disease virus containing a reticuloendotheliosis virus long terminal repeat
- Source :
- Avian Pathology. 45:657-666
- Publication Year :
- 2016
- Publisher :
- Informa UK Limited, 2016.
-
Abstract
- Marek’s disease virus (MDV), an alphaherpesvirus, causes Marek’s disease (MD), a lymphoproliferative disease in poultry characterized by T-cell lymphomas, nerve lesions, and mortality. Vaccination is used worldwide to control MD, but increasingly virulent field strains can overcome this protection, driving a need to create new vaccines. Previous studies revealed that insertion of reticuloendotheliosis virus (REV) long terminal repeat (LTR) into a bacterial artificial chromosome (BAC) clone of a very virulent strain of MDV, Md5, rendered the resultant recombinant virus, rMd5 REV-LTR BAC, fully attenuated in maternal antibody positive (Mab+) chickens at passage 40. In the current study, the protective efficacy of rMd5 REV-LTR BAC was evaluated. First, passage 70 was identified as being fully attenuated in maternal antibody negative chickens and chosen as the optimal passage level for use in protective efficacy studies. Second, three protective efficacy trials were conducted comparing the rMd5 REV-LTR p70 BAC to the CVI988/Rispens vaccine. Groups of Mab+ and Mab− 15I5 × 71 chickens were vaccinated in ovo at 18 days of embryonation or intra-abdominally at day of hatch, and challenged at 5 days post-hatch with the vv+MDV strain 686. Vaccination at day of hatch and in ovo with rMd5 REV-LTR p70 BAC protected chickens against MDV-induced bursa and thymic atrophy, but did not provide the same level of protection against MD tumours as that afforded by the commercial vaccine, CVI988/Rispens.
- Subjects :
- Male
0301 basic medicine
Chromosomes, Artificial, Bacterial
animal structures
viruses
DNA, Recombinant
Clone (cell biology)
Virulence
Biology
Antibodies, Viral
Recombinant virus
Virus
03 medical and health sciences
Food Animals
Marek Disease
Animals
Marek Disease Vaccines
Herpesvirus 2, Gallid
Cells, Cultured
Poultry Diseases
Bacterial artificial chromosome
Marek's disease
Reticuloendotheliosis virus
General Immunology and Microbiology
Vaccination
Terminal Repeat Sequences
High-Throughput Nucleotide Sequencing
Sequence Analysis, DNA
biology.organism_classification
Virology
Long terminal repeat
Ducks
030104 developmental biology
Female
Animal Science and Zoology
Chickens
Subjects
Details
- ISSN :
- 14653338 and 03079457
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Avian Pathology
- Accession number :
- edsair.doi.dedup.....d3fade11f6af752d7561c431c4c323f7