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Evaluation of Susceptibilities to Carbapenems and Faropenem Against Cephalosporin-Resistant Neisseria gonorrhoeae Clinical Isolates with penA Mosaic Alleles
- Source :
- Microbial drug resistance (Larchmont, N.Y.). 25(3)
- Publication Year :
- 2019
-
Abstract
- Neisseria gonorrhoeae is a principal pathogen for sexually transmitted infections, especially for male urethritis. Currently, the prevalence of multidrug resistance is increasing. Carbapenems are broad-spectrum antimicrobials that are widely used in the clinical setting, especially for multidrug-resistant Gram-negative bacteria. However, susceptibility to carbapenems has not been well evaluated for cephalosporin-resistant N. gonorrhoeae isolates. In this study, we determined the susceptibility to a series of carbapenems (meropenem, imipenem, doripenem, and biapenem) and faropenem against cephalosporin-resistant (resistant to cefixime, but susceptible to ceftriaxone) and cephalosporin-susceptible N. gonorrhoeae clinical isolates. The gene mutations associated with β-lactam resistance were evaluated. All cephalosporin-resistant N. gonorrhoeae isolates possessed mosaic mutation alleles in penA (NG-STAR penA-10.001, 27.001, or 108.001). They exhibited a low minimum inhibitory concentration (MIC) (≤0.125 mg/L) for meropenem and markedly high MICs (0.5-2 mg/L) for other carbapenems and faropenem. The strongest association was observed between the mosaic alleles in penA and decreased susceptibility to carbapenems and faropenem compared with mutations in mtrR, porB, and ponA. These results suggest that meropenem may serve as an alternative therapeutic agent for cephalosporin-resistant N. gonorrhoeae with a mosaic allele in penA, whereas other carbapenems and faropenem may be ineffective.
- Subjects :
- Microbiology (medical)
Male
Carbapenem
Penicillin binding proteins
Immunology
Microbial Sensitivity Tests
Biology
medicine.disease_cause
beta-Lactams
Microbiology
Meropenem
03 medical and health sciences
chemistry.chemical_compound
Bacterial Proteins
medicine
Humans
Urethritis
Pathogen
Alleles
030304 developmental biology
Pharmacology
0303 health sciences
Cephalosporin Resistance
030306 microbiology
Faropenem
medicine.disease
Neisseria gonorrhoeae
Anti-Bacterial Agents
Cephalosporins
Multiple drug resistance
chemistry
Carbapenems
Mutation
medicine.drug
Subjects
Details
- ISSN :
- 19318448
- Volume :
- 25
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Microbial drug resistance (Larchmont, N.Y.)
- Accession number :
- edsair.doi.dedup.....d3d8b6c75048b5955a6bfcdd0ffb48a8