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Expression of Cytochrome P450-4A Isoforms in the Rat Cremaster Muscle Microcirculation
- Source :
- Microcirculation. 11:89-96
- Publication Year :
- 2004
- Publisher :
- Wiley, 2004.
-
Abstract
- This study sought to identify any specific cytochrome P450 (CYP450) -4A enzyme isoforms expressed in arterioles and/or the surrounding parenchymal tissue of the rat cremaster muscle.RT-PCR was used to detect the presence of specific CYP450-4A isoforms in isolated muscle fibers and arterioles from the cremaster muscle of Sprague-Dawley rats; CYP450-4A protein expression was determined by Western blotting.CYP450-4A3 mRNA was expressed in isolated muscle fibers and in cremasteric arterioles, while CYP450-4A8 mRNA was expressed only in cremasteric arterioles. CYP450-4A1 and CYP450-4A2 mRNA were not expressed in arterioles and skeletal muscle cells, although all four isoforms were strongly expressed in the liver. CYP450-4A protein was detected in both the isolated muscle fibers and in the isolated arterioles.The present study identifies the specific pattern of cytochrome P450-4A isoform expression in arterioles and parenchymal cells of the skeletal muscle microcirculation, and supports the hypothesis that the cytochrome P-450 enzymes may play a role in the regulation of microvascular function in the skeletal muscle microcirculation.
- Subjects :
- Male
Gene isoform
Cytochrome
Physiology
Muscle Fibers, Skeletal
Microcirculation
Rats, Sprague-Dawley
Cytochrome P-450 Enzyme System
Physiology (medical)
Parenchyma
medicine
Animals
Tissue Distribution
Cytochrome P450 Family 4
RNA, Messenger
Muscle, Skeletal
Molecular Biology
Messenger RNA
biology
Cytochrome P450
Skeletal muscle
Molecular biology
Rats
Isoenzymes
Arterioles
medicine.anatomical_structure
Cremaster muscle
biology.protein
Cytochrome P-450 CYP4A
Cardiology and Cardiovascular Medicine
Subjects
Details
- ISSN :
- 15498719 and 10739688
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Microcirculation
- Accession number :
- edsair.doi.dedup.....d3cef21c23cb2e6fa6e970266ec21e46
- Full Text :
- https://doi.org/10.1080/10739680490266225