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Fibrin Biopolymer Incorporated with Antimicrobial Agents: A Proposal for Coating Denture Bases
- Source :
- Materials, Materials, Vol 14, Iss 1618, p 1618 (2021), Volume 14, Issue 7, Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP
- Publication Year :
- 2021
-
Abstract
- The characteristics of the denture base surface, in combination with the oral environment, promote the colonization and development of Candida albicans biofilm, which is the main cause of denture stomatitis. This study evaluated the effectiveness of fibrin biopolymer with digluconate chlorhexidine or Punica granatum alcoholic extract to prevent C. albicans biofilm. Conventional heat polymerized and pre-polymerized poly(methyl methacrylate) (PMMA) circular specimens (10 × 2 mm) were fabricated (n = 504) and randomly divided into groups: no treatment (control—CT), fibrin biopolymer coating (FB), fibrin biopolymer with P. granatum (FBPg), or digluconate of chlorhexidine (FBCh) coating. The specimens were inoculated with C. albicans SC5314 (1 × 107 cells/mL) and incubated for 24, 48, and 72 h. Crystal violet and colony-forming unit assays were used to quantify the total biofilm biomass and biofilm-living cells. A qualitative analysis was performed using confocal laser scanning microscopy. Data obtained are expressed as means and standard deviations and were statistically analyzed using a three-way analysis of variance (α = 0.05). The FBPg and FBCh groups inhibited the growth of C. albicans biofilm in both PMMA materials analyzed, with FBCh performing better in all periods evaluated (p &lt<br />0.0001). The colony forming unit (CFU) assay showed that the FB group favored the C. albicans biofilm growth at 24 h and 48 h (p &lt<br />0.0001), with no differences with CT group at 72 h (p = 0.790). All groups showed an enhancement in biofilm development up to 72 h (p &lt<br />0.0001), except the FBCh group (p = 0.100). No statistical differences were found between the PMMA base materials (p &gt<br />0.050), except in the FB group (p &lt<br />0.0001). Fibrin biopolymer, albeit a scaffold for the growth of C. albicans, when combined with chlorhexidine digluconate or P. granatum, demonstrated excellent performance as a drug delivery system, preventing and controlling the formation of denture biofilm.
- Subjects :
- acrylic resin
Acrylic resin
engineering.material
Complete denture
lcsh:Technology
CAD-CAM
Fibrin
Article
Denture stomatitis
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Candida albicans
medicine
General Materials Science
Crystal violet
lcsh:Microscopy
030304 developmental biology
lcsh:QC120-168.85
0303 health sciences
biology
lcsh:QH201-278.5
lcsh:T
Chlorhexidine
Biofilm
030206 dentistry
complete denture
biology.organism_classification
Corpus albicans
chemistry
lcsh:TA1-2040
denture stomatitis
visual_art
biology.protein
visual_art.visual_art_medium
engineering
lcsh:Descriptive and experimental mechanics
Biopolymer
lcsh:Electrical engineering. Electronics. Nuclear engineering
lcsh:Engineering (General). Civil engineering (General)
lcsh:TK1-9971
Nuclear chemistry
medicine.drug
Subjects
Details
- ISSN :
- 19961944
- Volume :
- 14
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Materials (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....d3cde8648f03c039b5060b266ef167df