Back to Search
Start Over
Avid binding by B cells to the Plasmodium circumsporozoite protein repeat suppresses responses to protective subdominant epitopes
- Source :
- Cell Reports, Vol 35, Iss 2, Pp 108996-(2021), Cell Reports
- Publication Year :
- 2020
- Publisher :
- Cold Spring Harbor Laboratory, 2020.
-
Abstract
- Summary Antibodies targeting the NANP/NVDP repeat domain of the Plasmodium falciparum circumsporozoite protein (CSPRepeat) can protect against malaria. However, it has also been suggested that the CSPRepeat is a decoy that prevents the immune system from mounting responses against other domains of CSP. Here, we show that, following parasite immunization, B cell responses to the CSPRepeat are immunodominant over responses to other CSP domains despite the presence of similar numbers of naive B cells able to bind these regions. We find that this immunodominance is driven by avid binding of the CSPRepeat to cognate B cells that are able to expand at the expense of B cells with other specificities. We further show that mice immunized with repeat-truncated CSP molecules develop responses to subdominant epitopes and are protected against malaria. These data demonstrate that the CSPRepeat functions as a decoy, but truncated CSP molecules may be an approach for malaria vaccination.<br />Graphical abstract<br />Highlights • The repeat domain is immunodominant within the circumsporozoite protein • High avidity responses by repeat-specific B cells inhibit subdominant responses • The number of naive B cell precursors does not predict immunodominance hierarchies • Vaccination with repeat-truncated circumsporozoite proteins induces robust protection<br />Chatterjee et al. show that avid B cell responses to repeating epitopes can suppress B cell responses to other regions of the same protein, driving immunodominance hierarchies. In the context of malaria vaccination, circumsporozoite-based immunogens carrying truncated repeat regions stimulated more diverse antibody responses and induced robust protection.
- Subjects :
- 0301 basic medicine
Adoptive cell transfer
Plasmodium berghei
Protozoan Proteins
Antibodies, Protozoan
Gene Expression
Epitope
Mice
0302 clinical medicine
immunodomination
Transgenes
Biology (General)
B-Lymphocytes
biology
Cell biology
Circumsporozoite protein
medicine.anatomical_structure
Sporozoites
malaria vaccines
Female
Antibody
circumsporozoite protein
Protein Binding
Subdominant
QH301-705.5
Naive B cell
Plasmodium falciparum
malaria
Immunodominance
Vaccines, Attenuated
Article
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Anopheles
parasitic diseases
medicine
Animals
B cell
B cells
immunodominance
fungi
biology.organism_classification
Antibodies, Neutralizing
Virology
Mice, Inbred C57BL
030104 developmental biology
biology.protein
Immunization
Peptides
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Cell Reports, Vol 35, Iss 2, Pp 108996-(2021), Cell Reports
- Accession number :
- edsair.doi.dedup.....d3ac7440ab47cc0879e8347e52c98927
- Full Text :
- https://doi.org/10.1101/2020.01.12.903682