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Apyap1affects aflatoxin biosynthesis duringAspergillus parasiticusgrowth in maize seeds
- Source :
- Food Additives and Contaminants, Food Additives and Contaminants, 2007, 24 (10), pp.1070-1075. ⟨10.1080/02652030701553244⟩, Food additives and contaminants 24 (2007): 1070–1075. doi:10.1080/02652030701553244, info:cnr-pdr/source/autori:Reverberi M., Zjalic S., Punelli F., Ricelli A., Fabbri A.A., Fanelli C./titolo:Apyap1 affects aflatoxin biosynthesis during Aspergillus parasiticus growth in maize seeds/doi:10.1080%2F02652030701553244/rivista:Food additives and contaminants/anno:2007/pagina_da:1070/pagina_a:1075/intervallo_pagine:1070–1075/volume:24
- Publication Year :
- 2007
- Publisher :
- Informa UK Limited, 2007.
-
Abstract
- It is demonstrated that, in fungal cells grown in synthetic media, the Apyap1 gene is implicated in the modulation of aflatoxin biosynthesis following the perturbation of redox balance. This study suggests that an association between oxidative stress and aflatoxin biosynthesis also occurs in maize seeds. We used DeltaApyap1, a strain in which the gene Apyap1 was disrupted, to verify whether this oxidative stress-related transcription factor, by affecting cell redox balance, can have a role in the modulation of aflatoxin synthesis. The amount of hydroperoxides (ROOH) produced by wild type (WT) and DeltaApyap1, both grown in potato dextrose broth, was assayed in the filtrate. In maize seeds (30 g), inoculated with WT and DeltaApyap1conidia and incubated at 30 degrees C for 15 days, lipoxygenase activity (LOX), lipoperoxides (LOOH) production, fungal growth and aflatoxin biosynthesis was analysed. It was observed that DeltaApyap1 released more hydroperoxides in the culture media and more aflatoxins in seeds, possibly through stronger stimulation of LOX, which, in turn led to greater LOOH production in the seeds. On the basis of the results, a hypothesis regarding strategies to control aflatoxin synthesis is formulated.
- Subjects :
- apyap1
Aflatoxin
Health, Toxicology and Mutagenesis
Genes, Fungal
Food Contamination
Toxicology
medicine.disease_cause
Zea mays
03 medical and health sciences
chemistry.chemical_compound
Aflatoxins
Biosynthesis
Gene Expression Regulation, Fungal
medicine
heterocyclic compounds
Mycotoxin
030304 developmental biology
0303 health sciences
biology
030306 microbiology
maize seeds
Public Health, Environmental and Occupational Health
Fungal genetics
Wild type
Life Sciences
food and beverages
General Chemistry
Fungi imperfecti
biology.organism_classification
Aspergillus parasiticus
aflatoxins
lipoperoxides
maize
aspergillus parasiticus
Oxidative Stress
Aspergillus
chemistry
Biochemistry
Chemistry (miscellaneous)
Seeds
aflatoxin biosynthesis
oxidative stress
Oxidative stress
Food Science
Subjects
Details
- ISSN :
- 0265203X
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Food Additives and Contaminants
- Accession number :
- edsair.doi.dedup.....d38d37ba7c551dd764a12a3f33df9528