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In Situ Detection of Plasma Exosomal MicroRNA-1246 for Breast Cancer Diagnostics by a Au Nanoflare Probe
- Source :
- ACS Applied Materials & Interfaces. 10:39478-39486
- Publication Year :
- 2018
- Publisher :
- American Chemical Society (ACS), 2018.
-
Abstract
- Breast cancer is the second cause of cancer mortality in women globally. Early detection, treatment, and metastasis monitoring are of great importance to favorable prognosis. Although conventional diagnostic methods, such as breast X-ray mammography and image positioning biopsy, are accurate, they could cause radioactive or invasive damage to patients. Liquid biopsy as a noninvasive method is convenient for repeated sampling in clinical cancer prognostic, metastatic evaluation, and relapse monitoring. MicroRNAs encased in exosomes circulating in biofluids are promising candidate cancer biomarkers because of their cancer-specific expression profiles. Here, we report an in situ detection of microRNA-1246 (miR-1246) in human plasma exosomes as breast cancer biomarker by a nucleic acid functionalized Au nanoflare probe. Needing neither time-consuming and costly isolation of exosomes from the plasma sample nor transfection means, the Au nanoflare probe can directly enter the plasma exosomes to generate fluorescent signal quantitatively by specifically targeting miR-1246. Only 40 μL of plasma is needed to incubate 4 h with the probe, giving signal sensitive enough to distinguish samples of breast cancer to normal control. Using plasma miR-1246 level detected by our assay as a marker, we differentiated 46 breast cancer patients from 28 healthy controls with 100% sensitivity and 92.9% specificity at the best cutoff. This simple, accurate, sensitive, and cost-effective liquid biopsy by the Au nanoflare probe is potent to be developed as a noninvasive breast cancer diagnostic assay for clinical adaption.
- Subjects :
- 0301 basic medicine
Materials science
Biopsy
Metal Nanoparticles
Breast Neoplasms
Exosomes
Sensitivity and Specificity
Metastasis
03 medical and health sciences
0302 clinical medicine
Breast cancer
Cell Line, Tumor
Biomarkers, Tumor
medicine
Humans
Mammography
General Materials Science
Liquid biopsy
medicine.diagnostic_test
Reproducibility of Results
Cancer
medicine.disease
Biomarker (cell)
Kinetics
MicroRNAs
030104 developmental biology
ROC Curve
030220 oncology & carcinogenesis
MCF-7 Cells
Cancer research
Thermodynamics
Female
Cancer biomarkers
Gold
Subjects
Details
- ISSN :
- 19448252 and 19448244
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- ACS Applied Materials & Interfaces
- Accession number :
- edsair.doi.dedup.....d37e53a816c40d164417297639dc3afa