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Notch activation promotes osteoblast mineralization by inhibition of apoptosis
- Source :
- Journal of Cellular Physiology. 233:6921-6928
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Notch activator Jagged1 (JAG1) plays a critical role in the regulation of osteoblast differentiation and bone metabolism. In this study, JAG1-induced osteoblast proliferation, differentiation, and mineralization has been analyzed in primary osteoblasts for up to 7 days. Alkaline phosphatase and Alizarin red staining showed an enhanced osteoblast maturation and mineralization in JAG1 treated cells, as well as higher mRNA levels of late osteoblast differentiation markers. In contrast, Notch inhibitor DAPT and deletion of Runx2 totally blocked JAG1 effects on osteoblast mineralization. Flow cytometry data further showed a significantly higher cell proliferation in early stages of culture at day 3, and lower levels of osteoblast apoptosis in late stages of culture at day 7. More importantly, activation of anti-apoptotic factor BCL-2 was enhanced, while pro-apoptotic factor Caspase3 was reduced in JAG1 treated osteoblasts. Therefore, we conclude that cell mineralization is enhanced via anti-apoptotic actions of Notch signaling within the osteoblast cells.
- Subjects :
- musculoskeletal diseases
0301 basic medicine
JAG1
Physiology
Clinical Biochemistry
Notch signaling pathway
Apoptosis
Mineralization (biology)
03 medical and health sciences
Calcification, Physiologic
Osteogenesis
medicine
Animals
Humans
Cells, Cultured
Cell Proliferation
Osteoblasts
Receptors, Notch
Chemistry
Cell growth
Cell Differentiation
Osteoblast
Cell Biology
Rats
Cell biology
RUNX2
030104 developmental biology
medicine.anatomical_structure
Alkaline phosphatase
Jagged-1 Protein
Signal Transduction
Subjects
Details
- ISSN :
- 10974652 and 00219541
- Volume :
- 233
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi.dedup.....d37a69eb0e157b35ad4b5f09acbe8a5a