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Short-Term In Vitro Effects of Low Frequency Ultrasound on Odontoblast-Like Cells
- Source :
- Ultrasound in Medicine & Biology. 33:1475-1482
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- In this study, the effects of low frequency ultrasound (US) were examined on odontoblasts, the primary cell responsible for dentinogenesis and dentine repair. An established odontoblast-like cell line, MDPC-23, was subjected to 30 kHz ultrasound at three different power settings. US induced a marginal level of cell death (3% to 4%) at lower amplitudes rising to 25% cell death at the highest power tested. The latter was reflected in a 30% decrease in cell attachment after 4 to 24 h of culture, while the number of adherent cells was reduced by approximately 10% to 15% in the lower power groups. Cell replication after 24 h, as measured by BrdU incorporation, showed no significant changes in the US-treated groups. Gene expression analyses demonstrated a moderate dose-dependent increase in the expression of GAPDH (glyseraldehyde-3-phosphate dehydrogenase)-normalised collagen type I, osteopontin (OPN), transforming growth factor-β1 (TGFβ1) and the heat shock protein (hsp) 70. The greatest change was found in the expression of the small hsp 25/27, which showed a two- to six-fold increase following US treatment. No significant effects were observed for alkaline phosphatase (ALP) and core-binding factor A1 (CBFA1/Runx2) expression levels. This is the first report describing US effects on odontoblasts. Further studies are warranted to elucidate US effects on odontoblast function and to evaluate US as a therapeutic application in dentine repair. (E-mail: b.a.scheven@bham.ac.uk )
- Subjects :
- Acoustics and Ultrasonics
Cell Survival
Cell
Biophysics
Gene Expression
Collagen Type I
Cell Line
Transforming Growth Factor beta1
Andrology
Mice
stomatognathic system
Cell Adhesion
medicine
Animals
Ultrasonics
Radiology, Nuclear Medicine and imaging
Osteopontin
Heat-Shock Proteins
Cell Death
Odontoblasts
Radiological and Ultrasound Technology
biology
Cell growth
Chemistry
Cell cycle
Odontoblast
medicine.anatomical_structure
Cell culture
Immunology
biology.protein
Dentinogenesis
Alkaline phosphatase
Biomarkers
Cell Division
Subjects
Details
- ISSN :
- 03015629
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Ultrasound in Medicine & Biology
- Accession number :
- edsair.doi.dedup.....d36cc0e1cd4230ab4181ec66b279a5fc