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Calreticulin functions in antimicrobial immunity of obscure puffer Takifugu obscurus
- Source :
- Molecular Immunology. 140:77-86
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Calreticulin (Crt) is a highly conserved and multi-functional protein with lectin-like properties and important immunological activities. In this study, a Crt homolog, namely, ToCrt, was cloned and characterized from the obscure puffer Takifugu obscurus with an open reading frame of 1278 bp encoding a putative protein of 425 amino acids. The deduced amino acid sequence of ToCrt consisted of three conserved structural domains: N-domain, P-domain, and C-terminal domain. In the phylogenetic tree, ToCrt formed a separate cluster with three Crts from other pufferfish species (Takifugu rubripes, Takifugu flavidus, and Takifugu bimaculatus). The mRNA transcript of ToCrt was ubiquitously expressed in all the examined tissues in a decreasing order: liver, spleen, kidney, gills, intestine, and heart. After Vibrio harveyi, Edwardsiella tarda, and Aeromonas hydrophila stimulations, the levels of ToCrt mRNA in the kidney and spleen were significantly upregulated compared with that in the control group. The recombinant calreticulin domain of ToCrt (rToCrt) could bind three Gram-negative bacteria (V. harveyi, E. tarda, and A. hydrophila) and polysaccharides from bacterial cell walls such as lipopolysaccharide and peptidoglycan. Meanwhile, rToCrt could agglutinate different kinds of microorganisms and exhibit antimicrobial activity. These results suggested that T. obscurus ToCrt could serve as an antimicrobial effector in the host immune response against invading microorganisms.
- Subjects :
- Agglutination
Time Factors
Takifugu rubripes
Immunology
Microbiology
chemistry.chemical_compound
Anti-Infective Agents
Protein Domains
Polysaccharides
Animals
Amino Acid Sequence
RNA, Messenger
Edwardsiella tarda
Molecular Biology
Peptide sequence
Phylogeny
Vibrio
Base Sequence
biology
Vibrio harveyi
Gene Expression Profiling
Immunity
biology.organism_classification
Aeromonas hydrophila
Takifugu
Open reading frame
Gene Expression Regulation
chemistry
biology.protein
Peptidoglycan
Calreticulin
Protein Binding
Subjects
Details
- ISSN :
- 01615890
- Volume :
- 140
- Database :
- OpenAIRE
- Journal :
- Molecular Immunology
- Accession number :
- edsair.doi.dedup.....d36b124aacd758fbf7171fd0a55a1527