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Cancer-Related Ischemic Stroke Has a Distinct Blood mRNA Expression Profile
- Source :
- Stroke
- Publication Year :
- 2019
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2019.
-
Abstract
- Background and Purpose— Comorbid cancer is common in patients with acute ischemic stroke (AIS). As blood mRNA profiles can distinguish AIS mechanisms, we hypothesized that cancer-related AIS would have a distinctive gene expression profile. Methods— We evaluated 4 groups of 10 subjects prospectively enrolled at 3 centers from 2009 to 2018. This included the group of interest with active solid tumor cancer and AIS and 3 control groups with active cancer only, AIS only, or vascular risk factors only. Subjects in the AIS-only and cancer-only groups were matched to subjects in the cancer-stroke group by age, sex, and cancer type (if applicable). Subjects in the vascular risk factor group were matched to subjects in the cancer-stroke and stroke-only groups by age, sex, and vascular risk factors. Blood was drawn 72 to 120 hours after stroke. Total RNA was processed using 3′ mRNA sequencing. ANOVA and Fisher least significant difference contrast methods were used to estimate differential gene expression between groups. Results— In the cancer-stroke group, 50% of strokes were cryptogenic. All groups had differentially expressed genes that could distinguish among them. Comparing the cancer-stroke group to the stroke-only group and after accounting for cancer-only genes, 438 genes were differentially expressed, including upregulation of multiple genes/pathways implicated in autophagy signaling, immunity/inflammation, and gene regulation, including IL (interleukin)-1, interferon, relaxin, mammalian target of rapamycin signaling, SQSTMI1 (sequestosome-1), and CREB1 (cAMP response element binding protein-1). Conclusions— This study provides evidence for a distinctive molecular signature in blood mRNA expression profiles of patients with cancer-related AIS. Future studies should evaluate whether blood mRNA can predict detection of occult cancer in patients with AIS. Clinical Trial Registration— URL: https://clinicaltrials.gov . Unique identifier: NCT02604667.
- Subjects :
- Male
Oncology
medicine.medical_specialty
Article
Brain Ischemia
03 medical and health sciences
0302 clinical medicine
Neoplasms
Internal medicine
Gene expression
Humans
Medicine
Prospective Studies
RNA, Messenger
RNA, Neoplasm
CAMP response element binding
Stroke
Aged
030304 developmental biology
Aged, 80 and over
Advanced and Specialized Nursing
Regulation of gene expression
0303 health sciences
biology
business.industry
Interleukin
Cancer
Middle Aged
medicine.disease
Neoplasm Proteins
Gene Expression Regulation, Neoplastic
MRNA Sequencing
biology.protein
Female
Neurology (clinical)
Transcriptome
Cardiology and Cardiovascular Medicine
business
CREB1
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15244628 and 00392499
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Stroke
- Accession number :
- edsair.doi.dedup.....d36387f8b1a13fd083b30c69c6ed53c6