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DNA Methylation Profiles of the DRD2 and NR3C1 Genes in Patients with Recent-Onset Psychosis

Authors :
Yan Hong Piao
Yin Cui
Ling Li
Maryam Karamikheirabad
Sung-Wan Kim
Bong Ju Lee
Jung Jin Kim
Je-Chun Yu
Kyu Young Lee
Seung-Hee Won
Seung-Hwan Lee
Seung-Hyun Kim
Shi Hyun Kang
Euitae Kim
Nam-In Kang
Fatima Zahra Rami
Young-Chul Chung
Source :
Disease Markers.
Publication Year :
2022
Publisher :
Hindawi, 2022.

Abstract

Objectives. Dopamine receptor D2 gene (DRD2) and glucocorticoid receptor gene (NR3C1) are implicated in the development of psychosis. We investigated methylation levels of DRD2 and NR3C1 in peripheral blood of patients with recent-onset (RO) psychosis using bisulfite pyrosequencing as well as its association with childhood trauma and rumination. Methods. In all, 51 individuals with RO psychosis and 47 healthy controls were recruited. DNA methylation levels in the targeted regions of two genes were analyzed and compared. Childhood trauma and rumination were evaluated using the Early Trauma Inventory Self-Report Short Form (ETI-SF) and Brooding Scale (BS), respectively. Correlations between the scores of the ETI-SF and BS and methylation levels were explored. Results. For DRD2, we found no significant differences between groups in terms of methylation level or association with childhood trauma or rumination. For NR3C1, we found a trend level significance for average value of all CpG sites and significant hypermethylation or hypomethylation at specific sites. There was also a significant positive correlation between the methylation level at the CpG8 site of NR3C1 exon 1F and negative symptom subscale score of the PANSS (PANSS-N). Conclusion. Epigenetic alterations of NR3C1 are associated with the pathophysiology of psychosis. Further epigenetic studies will elucidate the molecular mechanisms underpinning the pathophysiology of psychosis.

Details

Language :
English
ISSN :
02780240
Database :
OpenAIRE
Journal :
Disease Markers
Accession number :
edsair.doi.dedup.....d35b9bd4ef11cee223fd0b7888382df6
Full Text :
https://doi.org/10.1155/2022/2172564