Back to Search
Start Over
Interaction of brassinosteroid and cytokinin promotes ovule initiation and increases seed number per silique in Arabidopsis
- Source :
- Journal of Integrative Plant Biology. 64:702-716
- Publication Year :
- 2022
- Publisher :
- Wiley, 2022.
-
Abstract
- Ovule initiation is a key step that strongly influences ovule number and seed yield. Notably, mutants with enhanced brassinosteroid (BR) and cytokinin (CK) signaling produce more ovules and have a higher seed number per silique (SNS) than wild-type plants. Here, we crossed BR- and CK-related mutants to test whether these phytohormones function together in ovule initiation. We determined that simultaneously enhancing BR and CK contents led to higher ovule and seed numbers than enhancing BR or CK separately, and BR and CK enhanced each other. Further, the BR-response transcription factor BZR1 directly interacted with the CK-response transcription factor ARABIDOPSIS RESPONSE REGULATOR1 (ARR1). Treatments with BR or BR plus CK strengthened this interaction and subsequent ARR1 targeting and induction of downstream genes to promote ovule initiation. Enhanced CK signaling partially rescued the reduced SNS phenotype of BR-deficient/insensitive mutants whereas enhanced BR signaling failed to rescue the low SNS of CK-deficient mutants, suggesting that BR regulates ovule initiation and SNS through CK-mediated and CK-independent pathways. Our study thus reveals that interaction between BR and CK promotes ovule initiation and increases seed number, providing important clues for increasing the seed yield of dicot crops. This article is protected by copyright. All rights reserved.
- Subjects :
- Ovule
Cytokinins
biology
Arabidopsis Proteins
Mutant
Arabidopsis
food and beverages
Plant Science
biology.organism_classification
Biochemistry
General Biochemistry, Genetics and Molecular Biology
Cell biology
chemistry.chemical_compound
chemistry
Gene Expression Regulation, Plant
Brassinosteroids
Seeds
Cytokinin
Brassinosteroid
Silique
Transcription factor
Gene
Subjects
Details
- ISSN :
- 17447909 and 16729072
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Journal of Integrative Plant Biology
- Accession number :
- edsair.doi.dedup.....d343885503c0cd6252e643e29781ace7