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Histone H3 lysine 36 methylation affects temperature-induced alternative splicing and flowering in plants
- Source :
- Genome Biology, Vol 18, Iss 1, Pp 1-12 (2017), Genome Biology, Genome Biology, 18, Genome Biology 18 (2017)
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Background Global warming severely affects flowering time and reproductive success of plants. Alternative splicing of pre-messenger RNA (mRNA) is an important mechanism underlying ambient temperature-controlled responses in plants, yet its regulation is poorly understood. An increase in temperature promotes changes in plant morphology as well as the transition from the vegetative to the reproductive phase in Arabidopsis thaliana via changes in splicing of key regulatory genes. Here we investigate whether a particular histone modification affects ambient temperature-induced alternative splicing and flowering time. Results We use a genome-wide approach and perform RNA-sequencing (RNA-seq) analyses and histone H3 lysine 36 tri-methylation (H3K36me3) chromatin immunoprecipitation sequencing (ChIP-seq) in plants exposed to different ambient temperatures. Analysis and comparison of these datasets reveal that temperature-induced differentially spliced genes are enriched in H3K36me3. Moreover, we find that reduction of H3K36me3 deposition causes alteration in temperature-induced alternative splicing. We also show that plants with mutations in H3K36me3 writers, eraser, or readers have altered high ambient temperature-induced flowering. Conclusions Our results show a key role for the histone mark H3K36me3 in splicing regulation and plant plasticity to fluctuating ambient temperature. Our findings open new perspectives for the breeding of crops that can better cope with environmental changes due to climate change. Electronic supplementary material The online version of this article (doi:10.1186/s13059-017-1235-x) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Flowering time
lcsh:QH426-470
RNA Splicing
Arabidopsis
MADS Domain Proteins
Flowers
03 medical and health sciences
Histone H3
SDG8
Gene Expression Regulation, Plant
Laboratorium voor Moleculaire Biologie
Arabidopsis thaliana
BIOS Plant Development Systems
Ambient temperature
lcsh:QH301-705.5
Regulator gene
2. Zero hunger
Genetics
biology
Research
H3K36me3
fungi
Alternative splicing
Temperature
food and beverages
Histone-Lysine N-Methyltransferase
Methylation
DNA Methylation
biology.organism_classification
lcsh:Genetics
030104 developmental biology
Histone
lcsh:Biology (General)
Mutation
RNA splicing
biology.protein
Laboratory of Molecular Biology
EPS
Histone modification
Transcription Factors
Subjects
Details
- ISSN :
- 1474760X and 14747596
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Genome Biology
- Accession number :
- edsair.doi.dedup.....d3385e88850fbc47687ba5552a55be29