Changes in the mRNA transcripts of insulin-like growth factor ligand, receptors and binding proteins in bovine blastocysts and elongated embryos derived from somatic cell nuclear transfer

The objective of this study was to determine changes in the transcription of insulin-like growth factor (IGF)-related genes in blastocyst (BC)- and elongated (EL)-stage embryos produced by nuclear transfer using somatic cells (NT-SC). Bovine BC (day 7)- and EL (day 15)-stage embryos were obtained from NT-SC or in vivo production (Vivo). The relative abundance of mRNA was examined by RT- real-time PCR. The transcript of IGF-II was only detected at the EL stage in both the NT-SC and Vivo embryos. The level of transcription of the IGF-I receptor (r) in the NT-SC embryos was decreased at the EL stage and was significantly (P