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Identification of Rap1 as a Target for the Crk SH3 Domain-Binding Guanine Nucleotide-Releasing Factor C3G
- Source :
- Molecular and Cellular Biology. 15:6746-6753
- Publication Year :
- 1995
- Publisher :
- Informa UK Limited, 1995.
-
Abstract
- C3G, which was identified as a Crk SH3 domain-binding guanine nucleotide-releasing factor, shows sequence similarity to CDC25 and Sos family proteins (S. Tanaka, T. Morishita, Y. Hashimoto, S. Hattori, S. Nakamura, M. Shibuya, K. Matuoka, T. Takenawa, T. Kurata, K. Nagashima, and M. Matsuda, Proc. Natl. Acad. Sci. USA 91:3443-3447, 1994). The substrate specificity of C3G was examined by in vitro and in vivo experiments. C3G markedly stimulated dissociation of bound GDP from Rap1B but marginally affected the same reaction of other Ras family proteins (Ha-Ras, N-Ras, and RalA). C3G also stimulated binding of GTP-gamma S [guanosine 5'-3-O-(thio)triphosphate] to Rap1B. When C3G and Rap1A were expressed in COS7 cells, marked accumulation of the active GTP-bound form of Rap1A was observed, while Sos was not effective in the activation of Rap1A. These results clearly show that C3G is an activator for Rap1. Furthermore, expression of C3G with a membrane localization signal in a v-Ki-ras transformant, DT, induced a reversion of the cells to the flat form, possibly through the activation of endogenous Rap1.
- Subjects :
- Guanine
Molecular Sequence Data
Guanosine
Cell Cycle Proteins
Spodoptera
Biology
Transfection
Guanosine Diphosphate
Polymerase Chain Reaction
SH3 domain
Cell Line
Substrate Specificity
chemistry.chemical_compound
Adapter molecule crk
GTP-Binding Proteins
Guanine Nucleotide-Releasing Factor 2
Proto-Oncogene Proteins
Phosphoprotein Phosphatases
Animals
Guanine Nucleotide Exchange Factors
Molecular Biology
DNA Primers
Binding Sites
Base Sequence
ras-GRF1
Activator (genetics)
Membrane Proteins
Proteins
Cell Biology
Proto-Oncogene Proteins c-crk
Molecular biology
Recombinant Proteins
RALA
Kinetics
rap GTP-Binding Proteins
chemistry
Guanosine 5'-O-(3-Thiotriphosphate)
Protein Biosynthesis
Son of Sevenless Proteins
ras Guanine Nucleotide Exchange Factors
Rap1
Research Article
Subjects
Details
- ISSN :
- 10985549
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Biology
- Accession number :
- edsair.doi.dedup.....d323b2248ddb91b44afac38e2a32c6a3