Impact of single and mixed infections with Escherichia coli and Mycoplasma gallisepticum on Newcastle disease virus vaccine performance in broiler chickens: an in vivo perspective

Authors :: H.I. Mahmoud
Ahmed M. Erfan
B.A. Abdelrahman
Yousreya M Hashem
M.I. Abd El‐Hamid
Naglaa F.S. Awad
Source :: Journal of Applied Microbiology. 127:396-405
Publication Year :: 2019
Publisher :: Oxford University Press (OUP), 2019.
Abstract: AIMS: This study was designed to investigate, in an in vivo setting, the effects of single and combined infections with either Mycoplasma gallisepticum (MG) and/or Escherichia coli on the chicken immune response induced by Newcastle disease virus (NDV) vaccine. METHODS AND RESULTS: Humoral immunity was measured through detection of NDV antibody and anti‐NDV IgG titres using haemagglutination‐inhibition test and enzyme‐linked immunosorbent assay, respectively. In addition, the expression levels of pro‐inflammatory cytokines' genes (interleukin (IL) 6, IL4 and interferon (IFN) γ) were analysed using quantitative reverse transcription PCR. Significant (P

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