A Simple and Selective Analytical Procedure for the Extraction and Quantification of Lutein from Tomato By-Products by HPLC–DAD

Lutein, a yellow dihydroxylated carotenoid, is present in many dietary supplements due to its biological properties. Currently, lutein is extracted from marigold flowers by expensive and time-consuming processes. Since tomatoes contain significant levels of lutein, in this study we have examined the feasibility of using tomato by-products as an alternative, low-cost source of this carotenoid. The determination of this carotenoid was performed by highperformance liquid chromatography???diode array detection, after selective extraction from tomato waste product samples. Lutein levels ranged from 9.9 to 10.5 ??g/g dry weight. Tomato waste products may be indicated as an alternative commercial source of lutein for food fortification and/or dietary supplements.