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Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution
- Source :
- Nature methods
- Publication Year :
- 2019
-
Abstract
- We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables isotropic, subcellular resolution imaging with high optical sectioning capability and a large field of view over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and non-aqueous chemically cleared tissue preparations. Depending on the optical configuration, ctASLM provides up to 260 nm of axial resolution, a three to tenfold improvement over confocal and other reported cleared-tissue light-sheet microscopes. We imaged millimeter-scale cleared tissues with subcellular three-dimensional resolution, which enabled automated detection of multicellular tissue architectures, individual cells, synaptic spines and rare cell–cell interactions. Cleared-tissue axially swept light-sheet microscopy (ctASLM) enables high-speed, refraction index-independent imaging of live, cleared and expanded samples with isotropic, submicron resolution.
- Subjects :
- 0303 health sciences
Microscope
Materials science
Optical sectioning
Confocal
Isotropy
Cell Biology
Lateral resolution
Biochemistry
Article
law.invention
03 medical and health sciences
Mice
Microscopy, Fluorescence
law
Light sheet fluorescence microscopy
Microscopy
Animals
Molecular Biology
Zebrafish
030304 developmental biology
Biotechnology
Biomedical engineering
Clearance
Subjects
Details
- ISSN :
- 15487105
- Volume :
- 16
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Nature methods
- Accession number :
- edsair.doi.dedup.....d2e9c3fd844ae6587dab96d5f868880d