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ADAM15 Is an Adherens Junction Molecule Whose Surface Expression Can Be Driven by VE-Cadherin
- Source :
- Experimental Cell Research. 279:239-247
- Publication Year :
- 2002
- Publisher :
- Elsevier BV, 2002.
-
Abstract
- ADAM15 belongs to the family of proteins containing disintegrin and metalloprotease domains (ADAM) that have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules. Here we provide the first report on the localization of an ADAM in adherens junctions. We show that ADAM15 colocalizes with a cell adhesion molecule, vascular endothelial (VE)-cadherin, which mediates endothelial cell adherens junction formation. In contrast, the distribution of ADAM15 correlates poorly with the localization in cell contacts of one of its proposed ligands, the β1-integrin. Furthermore, ADAM15 accumulation in cell–cell contacts is preceded by VE-cadherin-mediated adherens junction formation. To investigate the dependence of ADAM15 surface expression on adherens junction formation, we coexpressed VE-cadherin with ADAM15 and an ADAM15 green fluorescence protein (GFP) fusion protein in Chinese hamster ovary cells. VE-cadherin coexpression results in the translocation of ADAM15–GFP to the cell periphery. Analysis of cell surface levels of ADAM15 and ADAM15–GFP, with or without VE-cadherin coexpression, clearly demonstrates that VE-cadherin can drive surface expression of ADAM15. Our data suggest that ADAM15 may be a novel component of adherens junctions and thus could play a role in endothelial functions that are mediated by these cell contacts.
- Subjects :
- ADAM15
Disintegrins
Recombinant Fusion Proteins
Green Fluorescent Proteins
Integrin
CHO Cells
Adherens junction
Antigens, CD
Cricetinae
Cell Adhesion
Disintegrin
Animals
Humans
Cytoskeleton
Integrin binding
biology
Cell adhesion molecule
Integrin beta1
Chinese hamster ovary cell
Membrane Proteins
Metalloendopeptidases
Adherens Junctions
Cell Biology
Cadherins
Immunohistochemistry
Actins
Cell biology
ADAM Proteins
Luminescent Proteins
Protein Transport
Catenin
biology.protein
Indicators and Reagents
Endothelium, Vascular
Subjects
Details
- ISSN :
- 00144827
- Volume :
- 279
- Database :
- OpenAIRE
- Journal :
- Experimental Cell Research
- Accession number :
- edsair.doi.dedup.....d2c551aab65d035ebafece140fb4864b