Back to Search
Start Over
High Throughput Phenotypic Selection of Mycobacterium tuberculosis Mutants with Impaired Resistance to Reactive Oxygen Species Identifies Genes Important for Intracellular Growth
- Source :
- PLoS ONE, PLoS ONE; Vol 8, PLoS ONE, Vol 8, Iss 1, p e53486 (2013)
- Publication Year :
- 2013
-
Abstract
- Mycobacterium tuberculosis has the remarkable capacity to survive within the hostile environment of the macrophage, and to resist potent antibacterial molecules such as reactive oxygen species (ROS). Thus, understanding mycobacterial resistance mechanisms against ROS may contribute to the development of new anti-tuberculosis therapies. Here we identified genes involved in such mechanisms by screening a high-density transposon mutant library, and we show that several of them are involved in the intracellular lifestyle of the pathogen. Many of these genes were found to play a part in cell envelope functions, further strengthening the important role of the mycobacterial cell envelope in protection against aggressions such as the ones caused by ROS inside host cells.
- Subjects :
- Bacterial Diseases
Mutant
lcsh:Medicine
medicine.disease_cause
Macrophage
lcsh:Science
Disease Resistance
chemistry.chemical_classification
0303 health sciences
Mutation
Multidisciplinary
Genomics
Phenotype
Bacterial Biochemistry
3. Good health
Host-Pathogen Interaction
Infectious Diseases
Host-Pathogen Interactions
Medicine
Intracellular
Research Article
Biology
Microbiology
Mycobacterium tuberculosis
03 medical and health sciences
Genetics
medicine
Humans
Tuberculosis
Tuberculosis, Pulmonary
Microbial Pathogens
Gene
030304 developmental biology
Reactive oxygen species
030306 microbiology
Macrophages
lcsh:R
Tropical Diseases (Non-Neglected)
Bacteriology
biology.organism_classification
High-Throughput Screening Assays
chemistry
Genes, Bacterial
lcsh:Q
Reactive Oxygen Species
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....d2c4c627a5c538ed9599fd0a4a8f3756
- Full Text :
- https://doi.org/10.1371/journal.pone.0053486