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Identification of nucleotide-excision-repair genes on human chromosomes 2 and 13 by functional complementation in hamster-human hybrids
- Source :
- Somatic Cell and Molecular Genetics. 13:539-551
- Publication Year :
- 1987
- Publisher :
- Springer Science and Business Media LLC, 1987.
-
Abstract
- The CHO UV-sensitive mutants UV24 and UV135 (complementation groups 3 and 5, respectively) are defective in nucleotide excision repair. After fusing each mutant with human lymphocytes, resistant hybrid clones showing genetic complementation were isolated by repeated exposure to UV radiation. Using a combination of isozyme markers, DNA probes, and cytogenetic methods to analyze the primary hybrids and their subclones, correction of the repair defect was shown to be correlated with the presence of a specific human chromosome in each case. Chromosome 2 corrected UV24, and the gene responsible was designated ERCC3. Line UV135 was corrected by human chromosome 13 and the gene designated ERCC5. The UV-sensitive mouse cell line, Q31, was shown not to complement UV135 and thus appears to be mutated in the same genetic locus (homologous to ERCC5) as UV135. Breakage of complementing chromosomes with retention of the genes correcting repair defects allowed the following provisional assignments: regional localization of ERCC5 to 13q14-q34, exclusion of ERCC3 from the region of chromosome 2 distal to p23, and relief of the ambiguity of ACP1 assignment (2p23 or 2p25) to 2p23 proximal to MDH1.
- Subjects :
- DNA Repair
Ultraviolet Rays
Hybrid Cells
Biology
medicine.disease_cause
Cricetulus
Gene mapping
Cricetinae
Genetics
Homologous chromosome
medicine
Animals
Humans
Chromosome 13
Mutation
Chromosomes, Human, Pair 13
Genetic Complementation Test
Chromosome Mapping
Chromosome
Cell Biology
General Medicine
Molecular biology
Complementation
Chromosomes, Human, Pair 2
Chromosome 22
Nucleotide excision repair
Subjects
Details
- ISSN :
- 15729931 and 07407750
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Somatic Cell and Molecular Genetics
- Accession number :
- edsair.doi.dedup.....d2bed64751cb97d2a6abbce8e9444676