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Preference Test of Plutella xylostella Larvae upon DMNT Treatment
- Source :
- Bio Protoc
- Publication Year :
- 2021
- Publisher :
- Bio-Protocol, LLC, 2021.
-
Abstract
- We describe a method to test the preference of insects in response to (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT). We use a device that includes a horizontal glass tube, two grooves (with activated carbon), air flow, rubber stoppers/tubes, transparent glass containers (optional), and a holder for the glass tube (optional). Equal amounts of activated carbon in the groove (removable) are placed at both ends to avoid air contamination. The air flow is generated by an air pump. In the closed device, different samples are placed at each end of the glass tube. The air pump at the top of the glass tube forms an air flow that converges to the middle site of the glass tube. In each test, insect larvae are located in the middle of the glass test tube. If the test samples release DMNT that can be sensed by insects, the insects will selectively move to one specific end of the glass tube. The number of insects that move to each end will be recorded for further studies. This method can also be used to test the preference of insects in response to other volatile compounds.
- Subjects :
- Strategy and Management
Mechanical Engineering
fungi
Airflow
Metals and Alloys
Industrial and Manufacturing Engineering
Natural rubber
Preference test
Air pump
visual_art
Methods Article
medicine
visual_art.visual_art_medium
Environmental science
Tube (container)
Composite material
Groove (music)
Glass tube
Activated carbon
medicine.drug
Subjects
Details
- ISSN :
- 23318325
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- BIO-PROTOCOL
- Accession number :
- edsair.doi.dedup.....d2a5901adcc96c39753a0e482adeaec1