Back to Search
Start Over
Pigment epithelium-derived factor negates oxidative stress in mouse oocytes
- Source :
- FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES. 35(6)
- Publication Year :
- 2021
-
Abstract
- Molecular changes, caused by various environmental factors, affect the quality and developmental potential of oocytes. Oxidative stress (OS) is a major factor involved in various gynecologic disorders and/or in aging. Recent studies suggest that elevated reactive oxygen species (ROS) hamper oocyte quality and future embryonic development. Pigment epithelium-derived factor (PEDF) is a pleiotropic protein, known for its antiangiogenic, anti-inflammatory, and antioxidative properties. Our previous findings demonstrate the antioxidative role of rPEDF in maintaining granulosa cell viability. In the current study, we examined the ability of PEDF to negate the adverse impact of OS on oocytes. Maturation rate of oocytes exposed to OS was significantly lower than that of control oocytes. The number of mtDNA copies in OS-exposed oocytes was significantly higher than in control oocytes (>3 times), whereas ATP concentration was significantly lower. Oocytes exposed to OS demonstrated impaired chromosome arrangement at the metaphase plate. PEDF significantly improved maturation rate of untreated OS-exposed oocytes. Moreover, mtDNA copy number, ATP concentration, and chromosome arrangement at the metaphase plate in rPEDF-treated OS-exposed oocytes were restored to the level of control oocytes. Our findings demonstrate that OS hampers the ability of oocytes to undergo proper in vitro maturation. The energetic balance of OS-exposed oocyte is characterized by excessive mtDNA replication and reduced ATP concentration; it hampers the ability of oocytes to perform high fidelity chromosome segregation. PEDF alleviates this damage, improves the rate of oocyte maturation, and preserves mtDNA level and ATP content, thus enabling oocytes to form proper metaphase plate and improve oocyte competence.
- Subjects :
- 0301 basic medicine
Granulosa cell
Embryonic Development
medicine.disease_cause
Biochemistry
DNA, Mitochondrial
Chromosome segregation
03 medical and health sciences
Mice
0302 clinical medicine
PEDF
Pregnancy
Genetics
medicine
Animals
Nerve Growth Factors
Eye Proteins
Molecular Biology
Serpins
chemistry.chemical_classification
Reactive oxygen species
Mice, Inbred ICR
Embryogenesis
Oocyte
In vitro maturation
Cell biology
In Vitro Oocyte Maturation Techniques
Mitochondria
Oxidative Stress
030104 developmental biology
medicine.anatomical_structure
chemistry
Oocytes
Female
Reactive Oxygen Species
030217 neurology & neurosurgery
Oxidative stress
Biotechnology
Subjects
Details
- ISSN :
- 15306860
- Volume :
- 35
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES
- Accession number :
- edsair.doi.dedup.....d2a1a2e00d72fecd4df7913886078a30