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Growth Inhibition Test for Identification of Mycoplasma Species Utilizing Dried Antiserum-Impregnated Paper Discs
- Publication Year :
- 1967
-
Abstract
- The growth inhibition test for identifying Mycoplasma species has been modified by drying antibody-impregnated paper discs at 5 C. When stored at −20 C, these discs have been found to retain their inhibitory activity for longer than 7 months. Since these discs can be stored for long period of time, significant advantages over present methods result. When, for example, discs are arranged on a ring, a single test can be used for the identification of an unknown human species. Valuable antisera can be distributed to other laboratories on paper discs in much less volume than can fluid antiserum. Considerable savings of time result from prior preparation of many discs that can then be stored and used over a long period of time. The growth-inhibiting antibody is stable, and the activity is not enhanced by a heat-labile accessory factor from fresh guinea pig serum which increases the antibody titer in the metabolic inhibition test.
- Subjects :
- Guinea Pigs
Mycoplasma species
medicine.disease_cause
Microbiology
Guinea pig
chemistry.chemical_compound
Mycoplasma
Antigen
medicine
Animals
Antigens
Molecular Biology
Antiserum
Bacteriological Techniques
Chromatography
biology
Immune Sera
fungi
Antibody titer
food and beverages
Taxonomy, Ecology, Morphology and Structure, and Microbiological Methods
chemistry
biology.protein
Antibody
Growth inhibition
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....d26e2a6cd577340aa4ba0252ef89b795