A Simple and Practical Method for Concentrating Blood Group Antibodies

Concentration of serum proteins, including blood group antibodies and complement components, is achieved rapidly and economically by dialysing the serum against dry Carbowax 20-M, a polyethylene glycol polymer with a molecular weight of 20,000. Such concentrates require no fluid or electrolyte readjustment for red cell testing and may be used for the identification of weak antibodies, the conversion of rare but unreliable antisera into useful laboratory reagents, and the preparation of serum specimens with high complement activity.