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Quantification and imaging of HER2 protein using nanocrystals conjugated with single-domain antibodies
- Source :
- Journal of Physics: Conference Series, Journal of Physics: Conference Series, 2017, International Symposium Physics, Engineering and Technologies for Bio-Medicine, 784, pp.012016. ⟨10.1088/1742-6596/784/1/012016⟩, Journal of Physics: Conference Series, IOP Publishing, 2017, International Symposium Physics, Engineering and Technologies for Bio-Medicine, 784, pp.012016. ⟨10.1088/1742-6596/784/1/012016⟩
- Publication Year :
- 2017
- Publisher :
- IOP Publishing, 2017.
-
Abstract
- International audience; This study dealt with quantification and imaging of human epidermal growth factor receptor 2 (HER2), an important prognostic marker for cancer diagnosis and treatment, using specific quantum-dot-based conjugates. Fluorescent inorganic nanocrystals or quantum dots (QDs) are extremely highly resistant to photobleaching and have a high emission quantum yield and a continuous range of emission spectra, from the ultraviolet to the infrared regions. Ultrasmall nanoprobes consisting of highly affine anti-HER2 single-domain antibodies (sdAbs or "nanobodies") conjugated with QDs in a strictly oriented manner have been designed. QDs with a fluorescence peak maxima at wavelengths of 562 nm, 569 nm, 570 nm or in the near-infrared region were used. Here, we present our results of ISA quantification of HER2 protein, in situ imaging of HER2 protein on the surface of HER2-positive SK-BR-3 cells in immunohistochemical experiments, and counting of stained with anti-HER2 conjugates HER2-positive SK-BR-3 cells in their mixture with unstained cells of the same culture in flow cytometry experiments. The data demonstrate that the anti-HER2 QD–sdAb conjugates obtained are highly specific and sensitive and could be used in numerous applications for advanced integrated diagnosis.
- Subjects :
- 0301 basic medicine
In situ
History
Materials science
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging
[SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
Quantum yield
Nanotechnology
02 engineering and technology
Conjugated system
Education
Flow cytometry
03 medical and health sciences
medicine
[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
skin and connective tissue diseases
[SDV.IB] Life Sciences [q-bio]/Bioengineering
medicine.diagnostic_test
021001 nanoscience & nanotechnology
Fluorescence
Photobleaching
3. Good health
Computer Science Applications
[SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging
030104 developmental biology
Quantum dot
Biophysics
[SDV.IB]Life Sciences [q-bio]/Bioengineering
0210 nano-technology
Conjugate
Subjects
Details
- ISSN :
- 17426596 and 17426588
- Volume :
- 784
- Database :
- OpenAIRE
- Journal :
- Journal of Physics: Conference Series
- Accession number :
- edsair.doi.dedup.....d249ddd835a7ee30ad776808b0e6a1b3