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Expression profiles of MMP-1 and TIMP-1 in lumbar intervertebral disc degeneration
- Source :
- Genetics and molecular research : GMR. 14(4)
- Publication Year :
- 2016
-
Abstract
- Lumbar intervertebral disc degeneration (IDD) is a common clinical pathology and has become a focus for research in recent years. Matrix metalloproteinases (MMPs) are enzymes responsible for the degradation of almost all extracellular matrix proteins (ECM). The over-expression of MMPs or tissue inhibitors of metalloproteinases (TIMPs) may disrupt the dynamic balance of the ECM. Therefore, in the current study, the expression levels of MMP-1 and TIMP-1 in lumbar IDD patients were evaluated in an attempt to elucidate their role in IDD pathogenesis and progression. In total, 60 IDD patients were recruited as the experimental group, along with 20 cases of lumbar vertebral injury without disc degeneration as the control group. Preoperative venous blood samples were collected, and intervertebral disc tissues were collected from the lesion during surgery. Serum and tissue levels of MMP-1 and TIMP-1 were quantified by enzyme-linked immunosorbent assay and immunohistochemical staining, respectively. Serum and tissue MMP-1 levels in IDD patients were significantly higher than those in the control group (P0.05). Additionally, sub-group analysis revealed that severe IDD patients had higher MMP-1 levels compared with mild or moderate IDD patients (P0.05). However, there were no significant differences in TIMP- 1 levels in either the serum or tissues of IDD patients compared to patients in the control group (P0.05). These results demonstrate that MMP-1 expression is increased in IDD, with higher expression observed in more severe cases, whereas TIMP-1 expression was similarly expressed in both normal and degenerated discs.
- Subjects :
- Adult
Male
medicine.medical_specialty
Pathology
Genotype
Intervertebral Disc Degeneration
Matrix metalloproteinase
Lesion
Extracellular matrix
Pathogenesis
Young Adult
Lumbar
Genetics
Medicine
Humans
Molecular Biology
Genetic Association Studies
Aged
Tissue Inhibitor of Metalloproteinase-1
Clinical pathology
business.industry
Lumbosacral Region
Intervertebral disc
General Medicine
Middle Aged
Radiography
medicine.anatomical_structure
Immunohistochemistry
Female
Matrix Metalloproteinase 3
medicine.symptom
Matrix Metalloproteinase 1
business
Subjects
Details
- ISSN :
- 16765680
- Volume :
- 14
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Genetics and molecular research : GMR
- Accession number :
- edsair.doi.dedup.....d248e6f6e4f7457900947633cff6c2fa