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SHUGOSHINs and PATRONUS protect meiotic centromere cohesion in Arabidopsis thaliana
- Source :
- The Plant journal : for cell and molecular biology. 77(5)
- Publication Year :
- 2013
-
Abstract
- In meiosis, chromosome cohesion is maintained by the cohesin complex, which is released in a two-step manner. At meiosis I, the meiosis-specific cohesin subunit Rec8 is cleaved by the protease Separase along chromosome arms, allowing homologous chromosome segregation. Next, in meiosis II, cleavage of the remaining centromere cohesin results in separation of the sister chromatids. In eukaryotes, protection of centromeric cohesion in meiosis I is mediated by SHUGOSHINs (SGOs). The Arabidopsis genome contains two SGO homologs. Here we demonstrate that Atsgo1 mutants show a premature loss of cohesion of sister chromatid centromeres at anaphase I and that AtSGO2 partially rescues this loss of cohesion. In addition to SGOs, we characterize PATRONUS which is specifically required for the maintenance of cohesion of sister chromatid centromeres in meiosis II. In contrast to the Atsgo1 Atsgo2 double mutant, patronus T-DNA insertion mutants only display loss of sister chromatid cohesion after meiosis I, and additionally show disorganized spindles, resulting in defects in chromosome segregation in meiosis. This leads to reduced fertility and aneuploid offspring. Furthermore, we detect aneuploidy in sporophytic tissue, indicating a role for PATRONUS in chromosome segregation in somatic cells. Thus, ploidy stability is preserved in Arabidopsis by PATRONUS during both meiosis and mitosis.
- Subjects :
- Genetics
Cohesin
Arabidopsis Proteins
Meiosis II
Centromere
Arabidopsis
Mitosis
Cell Cycle Proteins
Cell Biology
Plant Science
Spindle Apparatus
Biology
Chromosome segregation
Establishment of sister chromatid cohesion
Meiosis
Chromosome Segregation
Sister chromatids
Chromatid
Homologous chromosome segregation
Interkinesis
biological phenomena, cell phenomena, and immunity
Subjects
Details
- ISSN :
- 1365313X
- Volume :
- 77
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- The Plant journal : for cell and molecular biology
- Accession number :
- edsair.doi.dedup.....d205b49d66a57b1f760ad2599f6938a4