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Deer antler extract potentially facilitates xiphoid cartilage growth and regeneration and prevents inflammatory susceptibility by regulating multiple functional genes
- Source :
- Journal of Orthopaedic Surgery and Research, Journal of Orthopaedic Surgery and Research, Vol 16, Iss 1, Pp 1-13 (2021)
- Publication Year :
- 2021
-
Abstract
- Background Deer antler is a zoological exception due to its fantastic characteristics, including amazing growth rate and repeatable regeneration. Deer antler has been used as a key ingredient in traditional Chinese medicine relating to kidney and bone health for centuries. The aim of this study was to dissect the molecular regulation of deer antler extract (DAE) on xiphoid cartilage (XC). Methods The DAE used in this experiment was same as the one that was prepared as previously described. The specific pathogen-free (SPF) grade Sprague-Dawley (SD) rats were randomly divided into blank group (n =10) and DAE group (n =10) after 1-week adaptive feeding. The DAE used in this experiment was same as the one that was prepared as previously described. The rats in DAE group were fed with DAE for 3 weeks at a dose of 0.2 g/kg per day according to the body surface area normalization method, and the rats in blank group were fed with drinking water. Total RNA was extracted from XC located in the most distal edge of the sternum. Illumina RNA sequencing (RNA-seq) in combination with quantitative real-time polymerase chain reaction (qRT-PCR) validation assay was carried out to dissect the molecular regulation of DAE on XC. Results We demonstrated that DAE significantly increased the expression levels of DEGs involved in cartilage growth and regeneration, but decreased the expression levels of DEGs involved in inflammation, and mildly increased the expression levels of DEGs involved in chondrogenesis and chondrocyte proliferation. Conclusions Our findings suggest that DAE might serve as a complementary therapeutic regent for cartilage growth and regeneration to treat cartilage degenerative disease, such as osteoarthritis.
- Subjects :
- 0301 basic medicine
Male
lcsh:Diseases of the musculoskeletal system
Bone Regeneration
Gene Expression
Antlers
Osteoarthritis
Growth
Chondrocyte
Andrology
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Chondrocytes
lcsh:Orthopedic surgery
Medicine
Animals
Xiphoid cartilage
Regeneration
Orthopedics and Sports Medicine
Medicine, Chinese Traditional
Cell Proliferation
Inflammation
Kidney
business.industry
Tissue Extracts
Cartilage
Regeneration (biology)
Deer
RNA
Gene Expression Regulation, Developmental
Cell Differentiation
RNA sequencing
medicine.disease
Chondrogenesis
Antler
Deer antler extract
lcsh:RD701-811
030104 developmental biology
medicine.anatomical_structure
Molecular regulation
030220 oncology & carcinogenesis
Surgery
lcsh:RC925-935
Xiphoid Bone
business
Research Article
Subjects
Details
- ISSN :
- 1749799X
- Volume :
- 16
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of orthopaedic surgery and research
- Accession number :
- edsair.doi.dedup.....d20503613d07ab7af8faf98fbc9272ac