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Rb1 Protects Endothelial Cells from Hydrogen Peroxide-Induced Cell Senescence by Modulating Redox Status
- Source :
- Biological and Pharmaceutical Bulletin. 34:1072-1077
- Publication Year :
- 2011
- Publisher :
- Pharmaceutical Society of Japan, 2011.
-
Abstract
- Senescence of endothelial cells has been proposed to play an important role in endothelial dysfunction and atherogenesis. In the present study we aimed to investigate whether ginsenoside Rb1, a major constituent of ginseng, protects endothelial cells from H(2)O(2)-induced endothelial senescence. While H(2)O(2) induced premature senescent-like phenotype of human umbilical vein endothelial cells (HUVECs), as judged by increased senescence-associated β-galactosidase (SA-β-gal) activity, enlarged, flattened cell morphology and sustained growth arrest, our results demonstrated that Rb1 protected endothelial cells from oxidative stress induced senescence. Mechanistically, we found that Rb1 could markedly increase intracellular superoxide dismutase (Cu/Zn SOD/SOD1) activity and decrease the malondialdehyde (MDA) level in H(2)O(2)-treated HUVECs, and suppress the generation of intracellular reactive oxygen species (ROS). Consistent with these findings, Rb1 could effectively restore the protein expression of Cu/Zn SOD, which was down-regulated in H(2)O(2) treated cells. Taken together, our data demonstrate that Rb1 exhibits antioxidant effects and antagonizes H(2)O(2)-induced cellular senescence.
- Subjects :
- Senescence
Endothelium
Pharmaceutical Science
Real-Time Polymerase Chain Reaction
medicine.disease_cause
Cell morphology
Retinoblastoma Protein
Superoxide dismutase
Malondialdehyde
medicine
Humans
Endothelial dysfunction
Cells, Cultured
Cellular Senescence
DNA Primers
Pharmacology
chemistry.chemical_classification
Reactive oxygen species
Base Sequence
biology
Superoxide Dismutase
Free Radical Scavengers
Hydrogen Peroxide
General Medicine
medicine.disease
Cell biology
medicine.anatomical_structure
Biochemistry
chemistry
biology.protein
Human umbilical vein endothelial cell
Endothelium, Vascular
Oxidation-Reduction
Oxidative stress
Subjects
Details
- ISSN :
- 13475215 and 09186158
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- Biological and Pharmaceutical Bulletin
- Accession number :
- edsair.doi.dedup.....d1f720b671bdc62da4147b30704b442a