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Potential therapy of Fc-antigen combination-encoding DNA vaccination in mouse allergic airway inflammation
- Source :
- Clinical and experimental immunology. 154(1)
- Publication Year :
- 2008
-
Abstract
- Summary Vaccination with allergen-encoding DNA has been proposed as having potential for allergen-specific immunotherapy. In this study, we examine the therapeutic effect of allergen-encoding DNA vaccination directly to dendritic cells (DCs) on allergen-induced allergic airway inflammation in a mouse model and explore potential mechanism. Ovalbumin (OVA)-sensitized and challenged mice were immunized with DNA vaccine and received bronchoalveolar lavage (BAL) 1 day after the last challenge, to measure BAL levels of interleukin (IL)-4, IL-5, interferon (IFN)-gamma and differential cell count. Pulmonary DCs and Spleen DCs were purified and sorted according to the expression of CD11c+CD80+ and CD11c+CD86+ co-stimulatory molecules. Our data demonstrated that DNA vaccine therapy with OVA-Fc-pcDNA3·1 significantly prevented OVA-increased levels of IL-4, IL-5 and the percentage of eosinophils and OVA-decreased level of IFN-gamma. OVA-Fc-pcDNA3·1-treated mice had less severity of airway inflammation, and lower expression of CD11c+CD80+ and CD11c+CD86+ on pulmonary DCs, as compared with animals with OVA-pcDNA3·1, pcDNA3·1 and OVA respectively. DNA vaccine encoding both Fc and OVA was shown to be more effective than DNA vaccine encoding OVA alone. Our data indicate that Fc-antigen combination-encoding DNA vaccination has better preventive effects on antigen-induced airway inflammation by regulating DCs, and may be a new alternative therapy for asthma.
- Subjects :
- Male
Ovalbumin
medicine.medical_treatment
Immunology
chemical and pharmacologic phenomena
DNA vaccination
Interferon-gamma
Mice
Interferon
Vaccines, DNA
Immunology and Allergy
Medicine
Animals
Antigen-presenting cell
Mice, Inbred BALB C
biology
business.industry
Immunization, Passive
Interleukin
hemic and immune systems
Immunotherapy
Dendritic cell
Dendritic Cells
respiratory system
Immunoglobulin E
Flow Cytometry
Asthma
respiratory tract diseases
Immunoglobulin Fc Fragments
Vaccination
Eosinophils
biology.protein
Animal Studies
Interleukin-4
Interleukin-5
business
Bronchoalveolar Lavage Fluid
medicine.drug
Subjects
Details
- ISSN :
- 13652249
- Volume :
- 154
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Clinical and experimental immunology
- Accession number :
- edsair.doi.dedup.....d1d0c153e78f0cbb48c5ba7d549c8ac7