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Overcoming merohedral twinning in crystals of bacteriorhodopsin grown in lipidic mesophase

Authors :
Rouslan G. Efremov
Georg Büldt
Ekaterina Moiseeva
Valentin Borshchevskiy
Valentin Gordeliy
Source :
Acta Crystallographica Section D Biological Crystallography. 66:26-32
Publication Year :
2009
Publisher :
International Union of Crystallography (IUCr), 2009.

Abstract

Twinning is one of the most common crystal-growth defects in protein crystallography. There are neither efficient rational approaches for the growth of nontwinned protein crystals nor are there examples of systematic studies of the dependence of the twinning-ratio distribution on crystallization conditions. The description of the twinning phenomenon has been covered even less for membrane-protein crystals and is non-existent for crystals grown using lipidic phases (in meso). In the present work, possibilities for overcoming merohedral twinning are investigated for crystals of the membrane protein bacteriorhodopsin (bR) grownin meso. It is shown that traditional crystallization additives are not effective in the case of thein mesocrystallization of bR. The twinning ratio was determined for 310 crystals grown under different crystallization conditions. A correlation of the twinning ratio with the growth rate of the crystals was observed. Slow growth indicated that crystals had a noticeable chance of avoiding twinning. Model calculations were performed in order to rationalize this observation. The calculations confirmed the experimental observation that most crystals consist of two twin domains and showed that under this condition small changes in the probability of twin-domain formation lead to dramatic changes in the number of nontwinned crystals, which explains why slow crystal growth results in a considerable number of nontwinned crystals.

Details

ISSN :
09074449
Volume :
66
Database :
OpenAIRE
Journal :
Acta Crystallographica Section D Biological Crystallography
Accession number :
edsair.doi.dedup.....d1c8319a4b6c31e680cb56563de065e3
Full Text :
https://doi.org/10.1107/s0907444909042838