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Relationship between Enhanced Intensity of Contrast Enhanced Ultrasound and Microvessel Density of Aortic Atherosclerostic Plaque in Rabbit Model
- Source :
- PLoS ONE, PLoS ONE, Vol 9, Iss 4, p e92445 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science, 2014.
-
Abstract
- The aim of this study was to evaluate the relationship between enhanced intensity of contrast enhanced ultrasound and microvessel density of aortic atherosclerotic plaque in rabbit model. The abdominal aortas of thirty-six male New Zealand rabbits were damaged by balloon expansion and the animals were then fed a high fat diet for 12 weeks. Twenty-seven plaques on the near aortic wall were detected using conventional ultrasound examination. The maximum thickness of each plaque was recorded. CEUS was performed on these 27 plaques and the time-intensity curves (TICs) were analyzed offline. Using the quantitative ACQ software, features such as the arrival time (AT), time to peak (TTP), baseline intensity (BI), peak intensity (PI) and enhanced intensity (EI) (EI = PI-BI) were assessed. Inter- and intra-observer agreement of EI were assessed using the Bland-Altman test. After CEUS examination, the rabbits were sacrificed for pathological examination and CD34 monoclonal antibody immunohistochemical detection. Microvessel density (MVD) was counted under the microscope. The relationship between indexes of CEUS and the level of MVD was analyzed. There was a good positive linear correlation between EI and MVD (γ = 0. 854, P
- Subjects :
- Male
Pathology
medicine.medical_specialty
Cardiology
lcsh:Medicine
Histopathology
Contrast Media
Pathology and Laboratory Medicine
Research and Analysis Methods
Diet, High-Fat
Arrival time
Vascular Medicine
Diagnostic Radiology
Model Organisms
Diagnostic Medicine
medicine.artery
medicine
Medicine and Health Sciences
Animals
Cardiovascular Imaging
lcsh:Science
Aorta
Microvessel density
Ultrasonography
Multidisciplinary
business.industry
Radiology and Imaging
lcsh:R
Biology and Life Sciences
Animal Models
Atherosclerosis
Plaque, Atherosclerotic
Aortic wall
Intensity (physics)
Disease Models, Animal
Anatomical Pathology
Microvessels
Rabbit model
Cardiovascular Anatomy
Immunohistochemistry
lcsh:Q
Rabbits
Anatomy
business
Contrast-enhanced ultrasound
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....d1bbc100c6e04595a420db9b8b704ada