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Extrasynaptic NMDA receptor dependent long-term potentiation of hippocampal CA1 pyramidal neurons
- Source :
- Scientific Reports, Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- In the adult mouse hippocampus, NMDA receptors (NMDARs) of CA1 neurons play an important role in the synaptic plasticity. The location of NMDARs can determine their roles in the induction of long-term potentiation (LTP). However, the extrasynaptic NMDARs (ES-NMDARs) dependent LTP haven’t been reported. Here, through the use of a 5-Hz stimulation and MK-801 (an irreversible antagonist of NMDARs) in the CA1 neurons of adult mice hippocampal slices, synaptic NMDARs were selectively inhibited and NMDAR-mediated excitatory postsynaptic currents were not recovered. We found that a robust LTP was induced by 3-train 100-Hz stimulation when the synaptic NMDARs and extrasynaptic NR2B containing NMDARs were blocked, but not in the any of the following conditions: blocking of all NMDARs (synaptic and extrasynaptic), blocking of the synaptic NMDARs, and blocking of the synaptic NMDARs and extrasynaptic NR2A-containing NMDARs. The results indicate that this LTP is ES-NMDARs dependent, and NR2B-containing ES-NMDARs modulates the threshold of LTP induction.
- Subjects :
- Male
0301 basic medicine
Postsynaptic Current
Science
Long-Term Potentiation
Stimulation
Hippocampal formation
Receptors, N-Methyl-D-Aspartate
Article
Mice
03 medical and health sciences
0302 clinical medicine
mental disorders
LTP induction
Animals
CA1 Region, Hippocampal
Multidisciplinary
Chemistry
Pyramidal Cells
musculoskeletal, neural, and ocular physiology
Excitatory Postsynaptic Potentials
Long-term potentiation
Mice, Inbred C57BL
030104 developmental biology
nervous system
Anesthesia
Synaptic plasticity
Excitatory postsynaptic potential
Medicine
NMDA receptor
Dizocilpine Maleate
biological phenomena, cell phenomena, and immunity
Excitatory Amino Acid Antagonists
Neuroscience
psychological phenomena and processes
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....d1adb3ff3c739ea54e0129460aaee8c0