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Radiolabeled Arginine-Glycine-Aspartic Acid Peptides to Image Angiogenesis in Swine Model of Hibernating Myocardium
- Source :
- JACC: Cardiovascular Imaging. 1:500-510
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- ObjectivesOur aim was to image angiogenesis produced by endomyocardial injection of phVEGF165 in a swine model of hibernating myocardium using [123I]Gluco-arginine-glycine-aspartic acid (RGD) targeting the αvβ3 integrins.BackgroundA noninvasive test to monitor the efficacy of therapy inducing angiogenesis is needed. The interaction between extracellular matrix and endothelial cells in sprouting capillaries is effected primarily by αvβ3 integrins that bind through RGD motifs.MethodsAt 21 ± 4 days, after left circumflex coronary artery ameroid constrictor placement, 8 swine received endomyocardial injection of 1.2 mg phVEGF165 divided into 6 sites and 6 swine received saline (S) using nonfluoroscopic 3-dimensional endocardial mapping system (Noga)-guided delivery. After 20 ± 6 days, 13 animals were injected with 6.4 ± 1.7 mCi [123I]Gluco-RGD, 1 VEGF (vascular endothelial growth factor)-injected animal with I-123–labeled peptide control, and all animals with 2.5 ± 0.4 mCi of Tl-201 and underwent single-photon emission computed tomography imaging. Blood flow and echocardiographic measurements were made at both time points and tissue analyzed for fibrosis and capillary density by lectin staining.ResultsHibernating myocardium in the ameroid constrictor territory at time of injections was documented by reduced wall thickening compared with remote. Ratio of myocardial blood flow in left circumflex coronary artery/left anterior descending coronary artery territories increased by 15 ± 11% in the VEGF animals and fell 13 ± 12% in S-injected (p < 0.01). There was a small increase in wall thickening in constrictor territory after VEGF (8 ± 17%) while in S-injected animals wall thickening fell by 23 ± 31% (p = 0.01 vs. VEGF). Lectin staining as percent positive tissue staining for ameroid territory was higher in VEGF-injected compared with S-injected animals (2.5 ± 1.5% vs. 0.87 ± 0.52%, p = 0.01). Focal uptake of [123I]Gluco-RGD corresponding to Tl-201 defects was seen in VEGF-injected but not in S-injected animals. [123I]Gluco-RGD uptake in the ameroid territory as percent injected dose correlated with lectin staining (R2 = 0.80, p = 0.002).ConclusionsThese data suggest that single-photon emission computed tomography imaging of radiolabeled RGD peptides may be a useful noninvasive method to monitor therapy that induces angiogenesis in the heart.
- Subjects :
- Male
Vascular Endothelial Growth Factor A
Pathology
Time Factors
Swine
Angiogenesis
030204 cardiovascular system & hematology
Coronary Angiography
angiogenesis
chemistry.chemical_compound
0302 clinical medicine
Glucosides
myocardial hibernation
Hibernating myocardium
0303 health sciences
Ejection fraction
Gene Transfer Techniques
VEGF
Vascular endothelial growth factor
Vascular endothelial growth factor A
medicine.anatomical_structure
Radiology Nuclear Medicine and imaging
Echocardiography
medicine.symptom
Cardiology and Cardiovascular Medicine
Oligopeptides
medicine.medical_specialty
Neovascularization, Physiologic
Anterior Descending Coronary Artery
Article
03 medical and health sciences
Coronary circulation
cyclo-RGD peptides
Coronary Circulation
medicine
Animals
Humans
Radiology, Nuclear Medicine and imaging
030304 developmental biology
Tomography, Emission-Computed, Single-Photon
business.industry
Myocardium
Coronary Stenosis
Genetic Therapy
Blood flow
Integrin alphaVbeta3
Fibrosis
Capillaries
Disease Models, Animal
chemistry
integrins
Radiopharmaceuticals
business
Subjects
Details
- ISSN :
- 1936878X
- Volume :
- 1
- Database :
- OpenAIRE
- Journal :
- JACC: Cardiovascular Imaging
- Accession number :
- edsair.doi.dedup.....d19e723a19813c77ac75d0162ef8bd21