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Effects of anakinra on the small intestine mucositis induced by methotrexate in rats
- Source :
- Experimental Animals
- Publication Year :
- 2019
-
Abstract
- Intestinal mucositis is an important problem in the patients receiving cancer treatment. We aimed to investigate the effect of anakinra, which is a well known anti-oxidant and anti-inflammatory agent, on methotrexate-induced small intestine mucositis in rats. Forty rats were divided into 4 groups with 10 in each group. The healthy group (HG) and the methotrexate group (MTXG) were given distilled water, while the methotrexate + anakinra 50 (MTX+ANA50) and the methotrexate + anakinra 100 (MTX+ANA100) groups were intraperitoneally administered 50 and 100 mg/kg of anakinra. After one hour, the MTXG, MTX+ANA50 and MTX+ANA100 groups were given oral methotrexate at a dose of 5 mg/kg. This procedure was repeated once a day for 7 days. After the rats had been sacrificed, the small intestine tissue of rats were removed for the assesment of biochemical markers, histopathological evaluation and gene expression analyze. Statistical analyses of the data were performed using one-way ANOVA. Malondialdehyde (MDA), myeloperoxidase (MPO) and interleukin-6 (IL-6) levels were significantly higher, whereas total glutathione (tGSH) levels were significantly lower in MTXG (P
- Subjects :
- 0301 basic medicine
musculoskeletal diseases
Mucositis
medicine.medical_specialty
Original
medicine.disease_cause
Gastroenterology
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
Gene expression
Intestine, Small
Medicine
Animals
oxidative stress
rat
Anakinra
General Veterinary
biology
business.industry
General Medicine
medicine.disease
Malondialdehyde
Small intestine
Rats
Intestinal Diseases
030104 developmental biology
medicine.anatomical_structure
Methotrexate
chemistry
Myeloperoxidase
biology.protein
Animal Science and Zoology
business
030217 neurology & neurosurgery
Oxidative stress
medicine.drug
anakinra
Subjects
Details
- ISSN :
- 18817122
- Volume :
- 69
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Experimental animals
- Accession number :
- edsair.doi.dedup.....d19035195811049ec458020b07e3a803