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High Improvement in Lactic Acid Productivity by New Alkaliphilic Bacterium Using Repeated Batch Fermentation Integrated with Increased Substrate Concentration
- Source :
- BioMed Research International, Vol 2019 (2019), BioMed Research International
- Publication Year :
- 2019
- Publisher :
- Hindawi Limited, 2019.
-
Abstract
- Optically pure lactic acid (LA) is an important chemical platform that has a wide range of industrial and biotechnological applications. Improved parameters for cost effective LA production are of great interest for industrial developments. In the present study, an alkaliphilic lactic acid bacterium, BoM 1-2, was selected among 369 newly obtained bacterial isolates. It was characterized using API 50 CHL kit and identified asEnterococcus hiraeBoM 1-2 by 16S rRNA gene sequence analysis. Efficient polymer-gradeL-lactic acid production was achieved at pH 9.0 and 40°C. In batch fermentation strategy using 20 g L−1glucose, 19.6 g L−1lactic acid was obtained with volumetric productivity of 2.18 g L−1 h−1. While using 100 g L−1glucose, 96.0 g L−1lactic acid was obtained with volumetric productivity of 1.07 g L−1 h−1.The highest lactic acid concentration of 180.6 g L−1was achieved in multipulse fed batch strategy with volumetric productivity of 0.65 g L−1 h−1. To achieve higher productivity, repeated fermentation processes were applied using the two different strategies. In the first strategy, the lactic acid productivity was increased from 1.97 g L−1 h−1to 4.48 g L−1 h−1when the total of 10 repeated runs were carried out using 60 g L−1glucose, but lactic acid productivity decreased to 2.95 g L−1 h−1using 100 g L−1glucose. In second strategy, repeated fermentation coupled with gradual increase in glucose concentration from 40 to 100 g L−1was conducted for 24 runs. A dramatic increase in LA productivity up to 39.9 g L−1 h−1(18-fold compared to first run) was achieved using 40 g L−1glucose while volumetric productivity ranging between 24.8 and 29.9 g L−1 h−1was achieved using 60–100 g L−1glucose.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Article Subject
Batch fermentation
Polymers
lcsh:Medicine
01 natural sciences
General Biochemistry, Genetics and Molecular Biology
Substrate Specificity
03 medical and health sciences
chemistry.chemical_compound
Bioreactors
Enterococcus hirae
RNA, Ribosomal, 16S
010608 biotechnology
Biomass
Lactic Acid
Food science
General Immunology and Microbiology
biology
Chemistry
lcsh:R
General Medicine
biology.organism_classification
Substrate concentration
Lactic acid
Glucose
030104 developmental biology
Productivity (ecology)
Batch Cell Culture Techniques
Fermentation
Gradual increase
Bacteria
Research Article
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 23146141 and 23146133
- Volume :
- 2019
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....d1413addd919c288016174f2755cafdf