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Local cytokine profiles in knee osteoarthritis: elevated synovial fluid interleukin-15 differentiates early from end-stage disease
- Source :
- OSTEOARTHRITIS AND CARTILAGE, r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau, instname
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- Objective: Much of what is known about the inflammatory response in the synovial membrane (SM) of patients with osteoarthritis (OA) comes from studies of synovial tissues from end-stage disease. In this study, we sought to better characterize the inflammatory infiltrate in symptomatic patients with early signs of knee CA, and to determine how inflammatory cell populations relate to the pattern of cytokine and degradative enzyme production. Methods: Study populations comprised patients with degenerative meniscal tears and early cartilage thinning undergoing arthroscopic procedures (early CA) and patients undergoing total knee replacement for end-stage OA. Quantitative real-time polymerase chain reaction (PCR) was used to measure expression of SM cytolkines and enzymes implicated in the pathogenesis of inflammatory arthritis and CA, as well as cell lineage-specific markers. We quantified synovial fluid (SF) cytokines and enzymes by enzyme-linked immunosorbent assay (ELISA) and SM cell populations by immunohistochemistry. Results: We found increased levels of SF interleukin-15 (IL-15) protein in the early knee OA patients when compared to end-stage OA. Both SF IL-15 protein and numbers of CD8 cells within SM correlated with matrix metalloproteinase-1 (MMP-1) and three levels. TNF-alpha, IL-6 and IL-21 were also detectable in the SF of the majority of patients, and IL-15 levels were associated with IL-6 levels. Conclusion: IL-15 is elevated in early knee CA, suggesting activation of an innate immune response in the SM. The association of IL-15 expression with CD8 transcripts and MMPs implicates this cytokine in OA pathogenesis and as a candidate therapeutic target. (C) 2009 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
- Subjects :
- Cartilage, Articular
Male
Inflammatory arthritis
medicine.medical_treatment
Biomedical Engineering
Inflammation
Osteoarthritis
Severity of Illness Index
Rheumatology
Synovitis
Synovial Fluid
Synovium
medicine
Humans
Synovial fluid
Orthopedics and Sports Medicine
Aged
Interleukin-15
business.industry
Synovial Membrane
Middle Aged
Osteoarthritis, Knee
medicine.disease
Immunohistochemistry
medicine.anatomical_structure
Cytokine
Interleukin 15
Immunology
Cytokines
Synovial membrane
medicine.symptom
business
Biomarkers
Subjects
Details
- ISSN :
- 10634584
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Osteoarthritis and Cartilage
- Accession number :
- edsair.doi.dedup.....d1404269b9cee18b8c5dda7b49a45258